Quality-Check List for 5′ End and 3′ Tag Sequences
| N | No primer or restriction sites of the linker identified (no insert or inaccuracy sequence) |
| @ | Discrimination of ID-check marker |
| S | Very short sequence (truncated by inaccuracy) |
| C | Contamination (very similar to know E. coli or plasmid sequences) |
| R | Structural RNA contamination |
| M | Mitochondrial genomic sequences |
| L | Long poly A tail[i] |
| P | No or short poly(A) tail[i] |
| G | No or short G tail[ii] |
[i] Used only for 3′ sequences.
[ii] Used only for 5′ sequences.