Amino Acid Transport Activities of Synechocystis sp. Strain PCC 6803 and Some Mutants
| Substrate amino acid | Amino acid transport in strain PCC 6803[i] (nmol · min−1 · mg Chl−1) | Amino acid transport (% of the wild-type activity)[ii] | |||||||||
| CSMJ36 natD (slr0949) | CSMJ37 natE (slr1881) | CSMJ41 natC (sll0146) | CSMJ7 bgtB (sll1270) | CSMJ8 bgtA (slr1735) | CSMJ11 bgtB bgtA (sll1270 slr1735) | CSMJ24 natB bgtB (slr0559 sll1270) | CSMJ26 natB bgtA (slr0559 slr1735) | CSMJ30 natA bgtB (slr0467 sll1270) | CSMJ32 natA bgtA (slr0467 slr1735) | ||
| Basic | |||||||||||
| Arg | 426.7 | 90 | 92 | 86 | 3 | 3 | 3 | <1 | 3 | 1 | 1 |
| His | 6.2 | n.d.[iii] | n.d. | n.d. | 9 | 12 | 10 | 2 | 2 | 5 | 2 |
| Lys | 52.8 | 95 | 88 | 87 | <1 | <1 | <1 | <1 | <1 | <1 | <1 |
| Neutral | |||||||||||
| Ala | 10.2 | 1 | 1 | 1 | 80 | 91 | 97 | <1 | <1 | 1 | <1 |
| Gln | 5.9 | 98 | 86 | 89 | 19 | 21 | 22 | 2 | 2 | 3 | 3 |
| Gly | 9.4 | 1 | 1 | <1 | 80 | 93 | 89 | 1 | 1 | 2 | 1 |
| Leu | 7.7 | 2 | 1 | 1 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| Pro | 6.1 | 1 | 1 | 1 | 79 | 99 | 85 | <1 | <1 | <1 | 1 |
| Ser | 7.0 | 2 | 2 | 2 | 78 | 80 | 96 | 2 | 3 | 2 | 1 |
| Acidic | |||||||||||
| Glu | 60.9 | 87 | 98 | 71 | 99 | 74 | 97 | 89 | 95 | 85 | 84 |
[i] Data are the mean of 6–12 determinations with similar results (within 20% of the mean).
[ii] Two to four determinations were performed for each mutant, with similar results. For simplicity, the data are presented as a percent of the wild-type activity.
[iii] n.d., not determined.