Table 3.

Hox Genes Identified in the Forelimb and Hindlimb SAGE Libraries

Gene SAGE tag Tag count
forelimb hindlimb
Hoxa1 TCTGTAATAA 01
Hoxa7 AAGTGGAAGA 11
Hoxa9 AAACTGCTCT 52
Hoxa10 CATAAAAGGG 1310
Hoxa11 TGAAATAATA 01
Hoxa11,antisense1 CAATTGAGGC 97
Hoxa11,antisense1 CATCAGGGTA 04
Hoxa13 [ii] GTGGATTAAC 24
Hoxb8 CGCGCTGTGA 01
Hoxc9 TACGGCTCGC 02
Hoxc10 [iii] TAGCTTCCTT 04
CAAAGTTGAG 05
Hoxc11 [iv] TGCGTGAGTG 01
Hoxd10 [v] TTTCTGAAAA 10
Hoxd11 AGTCACTGTC 1715
Hoxd13 [ii] GGCCTCTCAG 63

[i] Unless otherwise noted, all SAGE tags in this table were initially identified by the ehmtag-mapping method and subsequently verified manually.1There are numerous polyadenylated antisense transcripts produced from this locus (Hsieh-Li et al. 1995). Both of these SAGE tags are valid matches to different antisense transcripts.

[ii] The SAGE tags for these genes were identified by cloning and sequencing the 3′ ends of the genes.

[iii] Obtained from an analysis of GenBank sequence data (GI:51413). There are two different poly(A) signals that would generate the two different SAGE tags listed here, the second of which was generated from an ATTAA poly(A) signal and poly(A) rich region 1 kb upstream (at 5752 bp) of the reported putative poly(A) signal (Peterson et al. 1992).

[iv] Obtained from the sequence presented in Figure 1 of Hostikka and Capecchi (1998).

[v] This SAGE tag was identified from a genomic clone (see Methods for details).