Table 1.

Kinetic Data of Enzymes Involved in Pyrosequencing

Enzyme K M(μM) k cat (S−1)
Klenow Polymerase[i] 0.18 (dTTP)0.92
ATP sulfurylase[ii] 0.56 (APS) 7.0 (PPi)38
Firefly luciferase[iii] 20 (ATP)0.015
Apyrase[iv] 120 (ATP) 260 (ADP)500  (ATP)

[i] Van Draanen et al. 1992.

[ii] Nyren and Lundin 1985.

[iii] DeLuca and McElroy 1984.

[iv] Traverso-Cori et al. 1965.