Reproducibility of the mRNA Quantification
| Sample preparation | Experiment | NF[i] | Increased or decreased[ii] | Fold change >2 or <–2[iii] |
| Same culture, | 1 | 0.98 | 1 | 0 |
| same RNA preparation, | 2 | 1.27 | 29 | 6[iii] |
| independent labeling and | 3 | 0.66 | 7 | 0 |
| hybridization | ||||
| Same culture, | 1 | 0.88 | 32 | 7 |
| independent RNA | 2 | 1.12 | 3 | 1 |
| preparation, labeling, and | 3 | 1.34 | 13 | 1 |
| hybridization | 4 | 1.70 | 3 | 0 |
| Different cultures | 1.22 | 3.33 | 61 |
[i] (NF) Normalization factor.
[ii] Transcripts called increased or decreased according to the Affymetrix GeneChip software.
[iii] Fold changes between −2.1 and −2.5.
[iv] Genes total = 1961.