(A) Alignment of 22 different 118 bp monomers of the centromeric satellite sequence. The first 4 monomers with names starting with 118 are cloned PCR products; the first clone was used as in situ probe in Figure 1C. The last 8 monomers, with names identical two by two except for the last letter, are extracted from the two ends of the same BAC clones. (B) Smith-Waterman alignment between the Tetraodon and the Fugu 118 bp repeat unit. The optimal alignment was obtained by comparing in forward and reverse orientation the Tetraodon sequence to a database of 118 versions of the Fugu monomer obtained by shifting the starting position by one base. (C) Alignment of 25 consecutive subtelocentric satellite monomers, together with the resulting consensus sequence. The only nonvariable base is the thymidine in fifth position.