Table 1.

Optimization of Parking Strategies with Overlap (G = 3 Gb;L = 150 kb)

Maximum allowed overlap (φ) Cost to reach 50% coverage[i] Number of gaps at 50% coverage Jamming limit Number of gaps at jamming limit
0.010870100000.747614950
0.11071092900.816711870
0.21068087420.88009620
0.31075082960.93627200
0.41090079210.98044170
0.511130759510
0.611460733510
0.711880715110
0.812410702710
0.913060695510
1.013860[ii] 694810

[i] One cost unit equals the cost of completely sequencing a 150 kb BAC. For example, if it cost $5000 to sequence one BAC, then it would cost (10870)($5000) ≈ $54 million to reach 50% coverage, permiting no overlap.

[ii] Assumes no screening.