Table 5.

Candidates for Horizontal Gene Transfer into N. crassa:Discontigs with Apparent Orthologs Only in Prokaryotes

Discontig Identification Prok E-value[i] SC E-value[ii] Euk E-value[iii]
IV. Cell/organism defense[iv]
  435catalase T (catalase-peroxidase)6 × 10−48  —[v] —[vi]
 1069nitrilase9 × 10−11 ——
V. Metabolism
   94salicylate 1-monooxygenase7 × 10−11 —3 × 10−5
  1632,5-DDOL dehydrogenase10−20 2 × 10−10 10−16  
  256[vii] polyketide synthase PKS12 × 10−17 —2 × 10−6
  351[vii] putative 2-nitropropane dioxygenase4 × 10−6 ——
  443putative amidohydrolase9 × 10−16 —0.006
  698cytochrome P-450nor2 4 × 10−14 ——
  935[vii] pyruvate-formulate lyase activating enzyme8 × 10−10 ——
  947meta-cleavage dioxygenase2 × 10−21 ——
Unclassified
  118hypothetical protein,B. subtilis 3 × 10−7 ——
  340ethylene-forming enzyme2 × 10−7 ——
 1179[viii] hypothetical protein, Synechocystissp.2 × 10−31 0.094—

[i] E-value of best BLAST hit for a prokaryotic organism in the nonfungal data set.

[ii] E-value of best BLAST hit in S. cerevisiae data set.

[iii] E-value of best BLAST hit to a eukaryotic organism in the nonfungal data set.

[iv] Functional categories are as described in Nelson et al. (1997), based on a modification of the system described by White and Kerlavage (1996).

[v] No S. cerevisiae database sequence received anE-value < 0.1.

[vi] No sequence from a eukaryotic organism in the nonfungal database received an E-value < 0.1.

[vii] These sequences have homologs in A. nidulans.

[viii] This sequence has an S. pombe homolog (E-value = 9 × 10−19).