F2 Pedigree Structure of the Initial Mating between DBA/2 (Male) and DU6i (Female)
| F2Subfamily | Number of offspring (male; female) | Number of offspring with serum parameters (male; female) |
| 1 (F1♂5 × F1♀17) | 32 (17; 15) | 32 (17; 15) |
| 2 (F1♂6 × F1♀18) | 34 (19; 15) | 33 (19; 14) |
| 3 (F1♂7 × F1♀19) | 26 (13; 13) | 25 (13; 12) |
| 4 (F1♂8 × F1♀20) | 45 (24; 21) | 43 (23; 20) |
| 5 (F1♂9 × F1♀21) | 25 (17; 8) | 24 (17; 7) |
| 6 (F1♂10 × F1♀22) | 28 (17; 11) | 14 (8; 6) |
| 7 (F1♂11 × F1♀23) | 20 (10; 10) | 20 (10; 10) |
| 8 (F1♂12 × F1♀24) | 51 (31; 20) | 32 (18; 14) |
| 9 (F1♂13 × F1♀25) | 38 (21; 17) | 37 (20; 17) |
| 10 (F1♂14 × F1♀26) | 50 (23; 27) | 49 (23; 26) |
| 11 (F1♂15 × F1♀27) | 37 (26; 11) | 37 (26; 11) |
| 12 (F1♂16 × F1♀28) | 25 (15; 10) | 24 (14; 10) |
| Total | 411 (233; 178) | 370 (208; 162) |