Table 3.

Varying Coverage

Cov Gap Clones Bury[iii](%) Largest (order)[iv] F+ clones[v]
10×18667662506 (4, 2, 0)13 (3), 11 (2)
20×313353743529 (17, 2, 0)33 (9), 18 (2)
30×020030795306 (15, 2, 0)54 (9), 32 (2)
40×026707837110 (15, 2, 0)72 (9), 44 (2)
50×033384859534 (37, 4, 0)93 (9), 57 (2)
Cov 1e-08 1e-10 1e-10, CpM[vi]
Ctgs F− F+ Qs Ctgs F− F+ Qs Ctgs F− Qs
10×1351442930 (1)193216282 (1)92804 (1)
20×486076190 (3)[vii] 95106652 (1)242518 (1)
30×22282621229 (4)515922845 (1)91181 (1)
40×1519524674 (3)343846562 (1)57365 (1)
50×12159411086 (3)2728778204 (1)43724 (1)

[i] (Cov) coverage; (CpM) cutoff plus marker; (Ctgs) contigs; (F−) false-negative; (F+) false-positive; (Qs) questionable.

[ii] The data set has perfect data, 145–185 kb clone lengths, a marker every 20 kb, and a tolerance of 7.

[iii] Percentage buried in which a buried clone has 90% or more shared bands with the parent clone.

[iv] The largest contig with no Q clones and its order triple.

[v] The number of clones that have an F+ overlap for the 1e-08 and 1e-10 cutoff, respectively. In parentheses is the number of regions that have F+ overlaps. The number of F+ overlaps is the same for 1e-10 with or without markers.

[vi] The CpM rules are (1 1e-08) (2 1e-07) (3 1e-06).

[vii] There are four chimeric contigs, but the fourth does not have Q clones. The three F+'s occur at the end of two contigs and bridge the contigs with little difficulty, i.e., F+'s at the ends of contigs causing an incorrect merge may result in a number of extra bands and gaps that looks the same as error in the data.