Schematic diagram of the L1Hs display and results. (A) L1 display protocol. A truncated or full-length L1Hs-Ta (rectangle) is depicted surrounded by flanking DNA (solid lines). The relative locations of the ACA and AccI sites are indicated. The dashed lines represent the products of two rounds of PCR amplifications. The arrows below indicate the relative positions and orientations of the arbitrary decamers and the 19- or 20-bp-long flanking primers that were synthesized to match the non-L1 flanking DNA sequences (3FPa, 3FPb, and 5FP). (B) L1 display results. A typical L1 display experiment performed with a single decamer on genomic DNA from six individuals is shown. The figure is an autoradiograph of the gel after Southern blotting and hybridization with oligonucleotide Hb. Ca-1 and 2, European/Caucasian 1 and 2; Ch, Chinese; Dr, Druse; Py, Pygmy; Me, Melanesian. The mobilities of the DNA size markers are indicated.