Evaluation of transcriptional capability of the potentialFGFR2 source Ya5 Alu element. The transcriptional efficiency of the de novo FGFR2 Alu repeat and its putative source gene were evaluated by Northern blot analysis from transient transfection studies. The following constructs were evaluated: (lane 1) p^-290Ap, (lane 2) p-⁴¹⁶Ya5NBC237, and (lane 3) p^NPYa5NBC237. Lanes 4 and5 are internal control only, and no DNA controls, respectively. Small arrows indicate the Alu transcripts and the open arrow indicates the internal control transcript. The ratio of the Alu transcript/control transcript (numbers below) was normalized to the p^NPYa5NBC237 transcription ratio, which was assigned the arbitrary value of 1.