Uterus Weight in Female +/+ and −/− R1 and C57 Mice at Different Ages and 17β-Estradiol and Progesterone Levels in R1 Animals
| Age (weeks) | +/+ | −/− | Percent reduction | t-test P-value |
| A. Uterus | ||||
| R1 | ||||
| 8 | 100.6 ± 29.7 (n = 8) | 83.3 ± 32.6 (n = 8) | N.R. | 0.285 |
| 9 | 113.2 ± 34.6 (n = 6) | 73.9 ± 26.4 (n = 6) | 35 | 0.051 |
| 10 | 114.9 ± 23.0 (n = 6) | 79.7 ± 27.8 (n = 7) | 31 | 0.032 |
| 24 | 162.7 ± 50.3 (n = 7) | 100.0 ± 44.8 (n = 7) | 39 | 0.030 |
| C57 | ||||
| 4 | 8.0 ± 2.9 (n = 7) | 7.1 ± 1.3 (n = 3) | N.R. | 0.599 |
| 6 | 82.5 ± 28.2 (n = 8) | 49.9 ± 5.2 (n = 7) | 40 | 0.010 |
| 8 | 98.9 ± 32.5 (n = 7) | 63.1 ± 8.9 (n = 5) | 36 | 0.040 |
| 10 | 94.5 ± 14.3 (n = 10) | 52.9 ± 20.1 (n = 7) | 44 | <0.001 |
| B. 17β-Estradiol (R1) | ||||
| 6 | 26.0 ± 15.6 (n = 7) | 19.8 ± 7.2 (n = 10) | 0.286 | |
| 8 | 27.3 ± 14.7 (n = 6) | 33.3 ± 18.8 (n = 8) | 0.536 | |
| 10 | 33.6 ± 22.1 (n = 8) | 32.9 ± 11.1 (n = 12) | 0.927 | |
| 24 | 39.8 ± 10.8 (n = 6) | 42.5 ± 18.2 (n = 4) | 0.776 | |
| C. Progesterone (R1) | ||||
| 6 | 6.2 ± 5.3 (n = 15) | 11.5 ± 12.4 (n = 13) | 0.144 | |
| 8 | 10.4 ± 12.7 (n = 5) | 4.0 ± 2.6 (n = 8) | 0.184 | |
| 10 | 4.0 ± 3.8 (n = 15) | 2.5 ± 3.3 (n = 5) | 0.533 | |
| 24 | 11.7 ± 8.6 (n = 9) | 6.7 ± 3.5 (n = 7) | 0.165 | |
[i] Uteri were collected at day 0.5 of vaginal plug except for the prepubertal uteri from 4-week-old virgin C57 mice. Sera were collected from virgin females for measurement of 17β-estradiol (pm/liter) and progesterone (nm/liter) levels. (N.R.) Not relevant, as P-value is insignificant.