---------
PTDSS2-201, PTDSS2|PB.8551.9
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(145, 145) and range(95, 95)
---------
---------
RNH1-201, RNH1|PB.8550.16
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 104, in process_gene
    tx_aln = TranscriptAlignment.from_transcripts(anchor, alternative)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 301, in from_transcripts
    return cls(anchor, other, events, anchor_events, anchor_blocks, other_events, other_blocks, event_to_block, block_to_events)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.TranscriptAlignment>", line 34, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 174, in __attrs_post_init__
    raise ValueError(f'TranscriptEvent lengths add up to {total_delta_nt}; expected {tx_length_diff}')
ValueError: TranscriptEvent lengths add up to 120; expected -916
---------
---------
LRRC56-201, LRRC56|PB.8553.1
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 569, in from_proteins
    match_tblock = one(interval.data for interval in intervals if interval.data.category is SeqAlignCat.MATCH)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/more_itertools/more.py", line 555, in one
    raise too_long or ValueError(msg)
ValueError: Expected exactly one item in iterable, but got M(1124:1296|1791:1963), M(923:1124|732:933), and perhaps more.
---------
---------
PHRF1-201, PHRF1|PB.8558.7
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(444, 444) and range(444, 444)
---------
---------
DEAF1-201, DEAF1|PB.8561.3
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(0, 0) and range(0, 0)
---------
---------
MRPL23-202, MRPL23|PB.8581.3
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 569, in from_proteins
    match_tblock = one(interval.data for interval in intervals if interval.data.category is SeqAlignCat.MATCH)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/more_itertools/more.py", line 555, in one
    raise too_long or ValueError(msg)
ValueError: Expected exactly one item in iterable, but got M(102:225|1942:2065), M(225:382|2717:2874), and perhaps more.
---------
---------
ILK-201, ILK|PB.8624.1
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/more_itertools/more.py", line 540, in one
    first_value = next(it)
                  ^^^^^^^^
StopIteration

The above exception was the direct cause of the following exception:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 553, in from_proteins
    tblock = one(interval.data for interval in intervals if interval.data.category in SEQ_DEL_INS)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/more_itertools/more.py", line 542, in one
    raise (
ValueError: too few items in iterable (expected 1)
---------
---------
RPL27A-201, RPL27A|PB.8637.10
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(0, 0) and range(0, 0)
---------
---------
RPL27A-201, RPL27A|PB.8637.4
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(48, 48) and range(48, 48)
---------
---------
RPS13-202, RPS13|PB.8688.4
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 569, in from_proteins
    match_tblock = one(interval.data for interval in intervals if interval.data.category is SeqAlignCat.MATCH)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/more_itertools/more.py", line 555, in one
    raise too_long or ValueError(msg)
ValueError: Expected exactly one item in iterable, but got M(49:98|207:256), M(98:177|438:517), and perhaps more.
---------
---------
KCNC1-201, KCNC1|PB.8695.1
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 104, in process_gene
    tx_aln = TranscriptAlignment.from_transcripts(anchor, alternative)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 301, in from_transcripts
    return cls(anchor, other, events, anchor_events, anchor_blocks, other_events, other_blocks, event_to_block, block_to_events)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.TranscriptAlignment>", line 34, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 174, in __attrs_post_init__
    raise ValueError(f'TranscriptEvent lengths add up to {total_delta_nt}; expected {tx_length_diff}')
ValueError: TranscriptEvent lengths add up to 1063; expected -1620
---------
---------
GTF2H1-201, GTF2H1|PB.8699.13
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/more_itertools/more.py", line 540, in one
    first_value = next(it)
                  ^^^^^^^^
StopIteration

The above exception was the direct cause of the following exception:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 553, in from_proteins
    tblock = one(interval.data for interval in intervals if interval.data.category in SEQ_DEL_INS)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/more_itertools/more.py", line 542, in one
    raise (
ValueError: too few items in iterable (expected 1)
---------
---------
EIF3M-208, EIF3M|PB.8733.19
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(0, 0) and range(0, 0)
---------
---------
NR1H3-211, NR1H3|PB.8800.3
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/more_itertools/more.py", line 540, in one
    first_value = next(it)
                  ^^^^^^^^
StopIteration

The above exception was the direct cause of the following exception:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 553, in from_proteins
    tblock = one(interval.data for interval in intervals if interval.data.category in SEQ_DEL_INS)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/more_itertools/more.py", line 542, in one
    raise (
ValueError: too few items in iterable (expected 1)
---------
---------
KBTBD4-202, KBTBD4|PB.8807.2
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(0, 0) and range(0, 0)
---------
---------
SLC43A1-201, SLC43A1|PB.8821.18
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 104, in process_gene
    tx_aln = TranscriptAlignment.from_transcripts(anchor, alternative)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 301, in from_transcripts
    return cls(anchor, other, events, anchor_events, anchor_blocks, other_events, other_blocks, event_to_block, block_to_events)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.TranscriptAlignment>", line 34, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 174, in __attrs_post_init__
    raise ValueError(f'TranscriptEvent lengths add up to {total_delta_nt}; expected {tx_length_diff}')
ValueError: TranscriptEvent lengths add up to -50; expected -1282
---------
---------
PATL1-201, PATL1|PB.8844.5
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(528, 528) and range(224, 224)
---------
---------
FADS2-202, FADS2|PB.8870.5
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(0, 0) and range(0, 0)
---------
---------
EML3-202, EML3|PB.8877.8
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 569, in from_proteins
    match_tblock = one(interval.data for interval in intervals if interval.data.category is SeqAlignCat.MATCH)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/more_itertools/more.py", line 555, in one
    raise too_long or ValueError(msg)
ValueError: Expected exactly one item in iterable, but got M(2805:3266|2778:3239), M(2784:2805|2677:2698), and perhaps more.
---------
---------
POLR2G-201, POLR2G|PB.8890.15
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(0, 0) and range(0, 0)
---------
---------
SF1-201, SF1|PB.8933.34
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(447, 447) and range(101, 101)
---------
---------
SF1-201, SF1|PB.8933.49
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(447, 447) and range(447, 447)
---------
---------
CDCA5-201, CDCA5|PB.8947.10
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 569, in from_proteins
    match_tblock = one(interval.data for interval in intervals if interval.data.category is SeqAlignCat.MATCH)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/more_itertools/more.py", line 555, in one
    raise too_long or ValueError(msg)
ValueError: Expected exactly one item in iterable, but got M(203:269|508:574), M(108:203|335:430), and perhaps more.
---------
---------
SYVN1-201, SYVN1|PB.8951.23
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(617, 617) and range(513, 513)
---------
---------
GAL3ST3-201, GAL3ST3|PB.8992.2
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(42, 42) and range(42, 42)
---------
---------
MRPL11-201, MRPL11|PB.9003.3
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(157, 157) and range(54, 54)
---------
---------
RBM14-RBM4-201, RBM14-RBM4|PB.9015.22
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 104, in process_gene
    tx_aln = TranscriptAlignment.from_transcripts(anchor, alternative)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 301, in from_transcripts
    return cls(anchor, other, events, anchor_events, anchor_blocks, other_events, other_blocks, event_to_block, block_to_events)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.TranscriptAlignment>", line 34, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 174, in __attrs_post_init__
    raise ValueError(f'TranscriptEvent lengths add up to {total_delta_nt}; expected {tx_length_diff}')
ValueError: TranscriptEvent lengths add up to 3364; expected 899
---------
---------
C11orf80-219, C11orf80|PB.9017.3
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(246, 246) and range(296, 296)
---------
---------
C11orf80-219, C11orf80|PB.9017.6
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(246, 246) and range(247, 247)
---------
---------
C11orf80-219, C11orf80|PB.9017.8
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(246, 246) and range(298, 298)
---------
---------
RAD9A-201, RAD9A|PB.9028.1
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 569, in from_proteins
    match_tblock = one(interval.data for interval in intervals if interval.data.category is SeqAlignCat.MATCH)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/more_itertools/more.py", line 555, in one
    raise too_long or ValueError(msg)
ValueError: Expected exactly one item in iterable, but got M(164:793|1743:2372), M(93:164|1250:1321), and perhaps more.
---------
---------
GSTP1-202, GSTP1|PB.9036.2
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(0, 0) and range(0, 0)
---------
---------
CHKA-202, CHKA|PB.9046.8
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 569, in from_proteins
    match_tblock = one(interval.data for interval in intervals if interval.data.category is SeqAlignCat.MATCH)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/more_itertools/more.py", line 555, in one
    raise too_long or ValueError(msg)
ValueError: Expected exactly one item in iterable, but got M(729:1755|887:1913), M(617:729|599:711), and perhaps more.
---------
---------
CHKA-202, CHKA|PB.9046.9
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(154, 154) and range(167, 167)
---------
---------
KMT5B-201, KMT5B|PB.9047.12
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(274, 274) and range(218, 218)
---------
---------
PPP6R3-203, PPP6R3|PB.9052.44
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(856, 856) and range(821, 821)
---------
---------
DHCR7-201, DHCR7|PB.9075.20
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(33, 33) and range(33, 33)
---------
---------
NADSYN1-201, NADSYN1|PB.9076.10
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(153, 153) and range(153, 153)
---------
---------
SPCS2-201, SPCS2|PB.9112.10
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(119, 119) and range(20, 20)
---------
---------
TSKU-201, TSKU|PB.9130.6
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 104, in process_gene
    tx_aln = TranscriptAlignment.from_transcripts(anchor, alternative)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 301, in from_transcripts
    return cls(anchor, other, events, anchor_events, anchor_blocks, other_events, other_blocks, event_to_block, block_to_events)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.TranscriptAlignment>", line 34, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 174, in __attrs_post_init__
    raise ValueError(f'TranscriptEvent lengths add up to {total_delta_nt}; expected {tx_length_diff}')
ValueError: TranscriptEvent lengths add up to 1143; expected -721
---------
---------
ALG8-201, ALG8|PB.9146.2
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(300, 300) and range(32, 32)
---------
---------
CEP57-207, CEP57|PB.9208.16
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 104, in process_gene
    tx_aln = TranscriptAlignment.from_transcripts(anchor, alternative)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 301, in from_transcripts
    return cls(anchor, other, events, anchor_events, anchor_blocks, other_events, other_blocks, event_to_block, block_to_events)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.TranscriptAlignment>", line 34, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 174, in __attrs_post_init__
    raise ValueError(f'TranscriptEvent lengths add up to {total_delta_nt}; expected {tx_length_diff}')
ValueError: TranscriptEvent lengths add up to 1711; expected 32
---------
---------
ACAT1-201, ACAT1|PB.9235.3
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(186, 186) and range(183, 183)
---------
---------
SLC37A4-201, SLC37A4|PB.9302.7
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(176, 176) and range(176, 176)
---------
---------
DPAGT1-201, DPAGT1|PB.9311.13
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(306, 306) and range(243, 243)
---------
---------
SORL1-201, SORL1|PB.9331.27
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(1315, 1315) and range(426, 426)
---------
---------
ZNF202-201, ZNF202|PB.9341.6
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(318, 318) and range(278, 278)
---------
---------
HYLS1-201, HYLS1|PB.9359.3
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 104, in process_gene
    tx_aln = TranscriptAlignment.from_transcripts(anchor, alternative)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 301, in from_transcripts
    return cls(anchor, other, events, anchor_events, anchor_blocks, other_events, other_blocks, event_to_block, block_to_events)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.TranscriptAlignment>", line 34, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 174, in __attrs_post_init__
    raise ValueError(f'TranscriptEvent lengths add up to {total_delta_nt}; expected {tx_length_diff}')
ValueError: TranscriptEvent lengths add up to 394; expected -708
---------
---------
RPUSD4-201, RPUSD4|PB.9363.4
Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 216, in get_cblocks
    dfs[chr] = pd.read_csv(df_file, sep='\t')
               ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 912, in read_csv
    return _read(filepath_or_buffer, kwds)
           ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 577, in _read
    parser = TextFileReader(filepath_or_buffer, **kwds)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1407, in __init__
    self._engine = self._make_engine(f, self.engine)
                   ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/parsers/readers.py", line 1661, in _make_engine
    self.handles = get_handle(
                   ^^^^^^^^^^^
  File "/Users/mayankmurali/miniconda3/envs/biosurfer-install/lib/python3.11/site-packages/pandas/io/common.py", line 859, in get_handle
    handle = open(
             ^^^^^
FileNotFoundError: [Errno 2] No such file or directory: 'B_hybrid_aln_wtc11/cblock-tables/cblocks-chr11.tsv'

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/analysis/genome_wide_alignment_analysis.py", line 105, in process_gene
    cd_aln = CodonAlignment.from_proteins(anchor.protein, alternative.protein)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 523, in from_proteins
    cblock = CodonAlignmentBlock(range(anchor_start, anchor_stop), range(other_start, other_stop), category=category)
             ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
  File "<attrs generated init biosurfer.core.alignments.CodonAlignmentBlock>", line 12, in __init__
    self.__attrs_post_init__()
  File "/Users/mayankmurali/Desktop/Desktop - Mayank’s MacBook Pro/biosurfer_analysis_test_2/biosurfer/biosurfer/core/alignments.py", line 46, in __attrs_post_init__
    raise ValueError(f'Invalid ranges {self.anchor_range} and {self.other_range}')
ValueError: Invalid ranges range(186, 186) and range(186, 186)
---------
