02_sgRNA-level-analysis

There are three different subdirectories in the directory. dat contains files which are necessary to execute the scripts in the directory, and figures contains generated figures by the scripts. scripts-for-analysis contains snippets to parse and execute CB2.

*.R files are executable files and you can execute the scripts in Rstudio, or run Rscript <file-name-of-the-script.R. The following table describles the function of each script and what will be generated by the script.

File name Executable Function
draw-fig-S12.R Yes The script to generate FPR plots of non-targeting sgRNA analysis using Sanson et al.’s data.
draw-fig-S13.R Yes The script to create Heatmaps and Venn diagrams to reveal discrepancy of sgRNA statistics between CB2 and MAGeCK
draw-fig-S15.R Yes The script to create mean-variance plots to claim CRISPR pooled data can be under/over-dispersed.
draw-fig3.R Yes The script to generate FPR plots of non-targeting sgRNA analysis using Parnas et al.’s data.
draw-fig4.R Yes The script to create Heatmaps and Venn diagrams to reveal discrepancy of sgRNA statistics between CB2 and MAGeCK.