broad.core.primer3.RestrictionEnzyme Class Reference

Collaboration diagram for broad.core.primer3.RestrictionEnzyme:

Public Member Functions
	RestrictionEnzyme (String name, String restrictionSite, int fivePrimeCutFromSite, int threePrimerCutFromSite)
List< String >	digest (String seq)
String	getName ()

Static Public Attributes
static String[][]	WOBLE_PATTERN_MAP
static RestrictionEnzyme	AcuI = new RestrictionEnzyme("AcuI", "CTGAAG", 17, 15)
static RestrictionEnzyme	BpmI = new RestrictionEnzyme("BpmI", "CTGGAG", 16, 14)
static RestrictionEnzyme	BpuEI = new RestrictionEnzyme("BpuEI", "CTTGAG", 16, 14)
static RestrictionEnzyme	BsgI = new RestrictionEnzyme("BsgI", "GTGCAG", 16, 14)
static RestrictionEnzyme	EcoP15I = new RestrictionEnzyme("EcoP15I", "CAGCAG", 27, 27)
static RestrictionEnzyme	MmeI = new RestrictionEnzyme("MmeI", "TCCRAC", 20, 18)
static RestrictionEnzyme	BbvI = new RestrictionEnzyme("BbvI", "GCAGC", 8, 12)
static RestrictionEnzyme	BceAI = new RestrictionEnzyme("BceAI", "ACGGC", 12, 14)
static RestrictionEnzyme	BsmFI = new RestrictionEnzyme("BsmFI", "GGGAC", 10, 14)
static RestrictionEnzyme	BtgZI = new RestrictionEnzyme("BtgZI", "GCGATG", 10, 14)
static RestrictionEnzyme	EciI = new RestrictionEnzyme("EciI", "GGCGGA", 11, 9)
static RestrictionEnzyme	FokI = new RestrictionEnzyme("FokI", "GGATG", 9, 13)

Constructor & Destructor Documentation

broad.core.primer3.RestrictionEnzyme.RestrictionEnzyme	(	String	name,
		String	restrictionSite,
		int	fivePrimeCutFromSite,
		int	threePrimerCutFromSite
	)

Member Function Documentation

List<String> broad.core.primer3.RestrictionEnzyme.digest

(

String

seq

)

String broad.core.primer3.RestrictionEnzyme.getName

(

)

Member Data Documentation

RestrictionEnzyme broad.core.primer3.RestrictionEnzyme.AcuI = new RestrictionEnzyme("AcuI", "CTGAAG", 17, 15)

static

RestrictionEnzyme broad.core.primer3.RestrictionEnzyme.BbvI = new RestrictionEnzyme("BbvI", "GCAGC", 8, 12)

static

RestrictionEnzyme broad.core.primer3.RestrictionEnzyme.BceAI = new RestrictionEnzyme("BceAI", "ACGGC", 12, 14)

static

RestrictionEnzyme broad.core.primer3.RestrictionEnzyme.BpmI = new RestrictionEnzyme("BpmI", "CTGGAG", 16, 14)

static

RestrictionEnzyme broad.core.primer3.RestrictionEnzyme.BpuEI = new RestrictionEnzyme("BpuEI", "CTTGAG", 16, 14)

static

RestrictionEnzyme broad.core.primer3.RestrictionEnzyme.BsgI = new RestrictionEnzyme("BsgI", "GTGCAG", 16, 14)

static

RestrictionEnzyme broad.core.primer3.RestrictionEnzyme.BsmFI = new RestrictionEnzyme("BsmFI", "GGGAC", 10, 14)

static

RestrictionEnzyme broad.core.primer3.RestrictionEnzyme.BtgZI = new RestrictionEnzyme("BtgZI", "GCGATG", 10, 14)

static

RestrictionEnzyme broad.core.primer3.RestrictionEnzyme.EciI = new RestrictionEnzyme("EciI", "GGCGGA", 11, 9)

static

RestrictionEnzyme broad.core.primer3.RestrictionEnzyme.EcoP15I = new RestrictionEnzyme("EcoP15I", "CAGCAG", 27, 27)

static

RestrictionEnzyme broad.core.primer3.RestrictionEnzyme.FokI = new RestrictionEnzyme("FokI", "GGATG", 9, 13)

static

RestrictionEnzyme broad.core.primer3.RestrictionEnzyme.MmeI = new RestrictionEnzyme("MmeI", "TCCRAC", 20, 18)

static

String [][] broad.core.primer3.RestrictionEnzyme.WOBLE_PATTERN_MAP

static

Initial value:

= {
        {"M",  "[AC]"},
        {"R",  "[AG]"},
        {"S",  "[CG]"},
        {"V",  "[AGC]"},
        {"W",  "[AT]"},
        {"Y",  "[CT]"},
        {"H",  "[ACT]"},
        {"K" , "[GT]"},
        {"D" , "[AGT]"},
        {"B" , "[TGC]"},
        {"N" , "[ATGC]"}
    }

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The documentation for this class was generated from the following file:

• java/broad/core/primer3/RestrictionEnzyme.java