sample # 23 44 45 46 47 59 60 62 3 8 24 25 26 27 28 30 32 33 34 35 36 37 49 50 51 52 54 55 63 64 65 66 69 72 73 74 77 78 80 82 84 86 118 119 120 121 122 123 124 125 134 135 136 126 127 129 130 132 133 142 143 146 Vector Only PCNE ID 5596-Bf_ECR1_C1 4881-Dr_ECR1_C11 4881-Dr_ECR1_C12 5596-Dr_ECR3_C1 5596-Dr_ECR7_C1 4881-Tr_ECR5_C11 4881-Tr_ECR3_C12 5596-Tr_ECR5_C1 3352-Bf_ECR32_C5 67-Bf_ECR20_C2 496-Bf_ECR3_C3 4517-Bf_ECR2_C4 2791-Bf_ECR5_C4 2791-Bf_ECR10_C1 4562-Bf_ECR2_C1 5672-Bf_ECR3_C12 5694-Bf_ECR6_C6 5694-Bf_ECR7_C14 5882-Bf_ECR13_C2 5882-Bf_ECR12_C2 3062-Bf_ECR8_C3 67-Bf_ECR4_C4 3621-Bf_ECR11_C2 3643-Bf_ECR1_C1 5672-Bf_ECR26_C2 5694-Bf_ECR5_C3 5694-Dr_ECR1_C6 5694-Dr_ECR1_C14 5672-Tr_ECR4_C12 67-Dr_ECR2_C4 67-Dr_ECR7_C2 2791-Dr_ECR2_C1 3703-Bf_ECR2_C8 6281-Bf_ECR2_C1 5157-Bf_ECR2_C2 5157-Bf_ECR18_C1 3958-Bf_ECR24_C1 3958-Bf_ECR7_C3 5349-Bf_ECR3_C2 6407-Bf_ECR5_C5 6436-Bf_ECR2_C1 6456-Bf_ECR2_C1 4517-Tr_ECR5_C4 496-Tr_ECR1_C3 2791-Tr_ECR4_C4 3621-Tr_ECR7_C2 3643-Tr_ECR4_C1 5157-Tr_ECR1_C2 5694-Tr_ECR20_C3 5694-Tr_ECR6_C14 8085-Bf_ECR1_C1 2031-Bf_ECR1_C2 5848-Bf_ECR2_C1 rand_602-215234-215293 rand_2286-4103-4162 rand_1452-2928-2987 rand_2370-6155-6214 rand_1028-6323-6382 rand_2889-297-356 rand_16-46219015-46219064 rand_12-46392970-46393019 rand_8-31645402-31645451 Parent Gene SoxB2 Sox2 Sox2 Sox21b Sox14 Sox2 Sox2 Sox21 Pax6 Ptpr S/D/F myosin III Sp5 ELAV-like ELAV-like POU6 lmo1/3 BarH-like BarH-like muscleblind muscleblind shh Ptpr S/D/F Six3/6 Six1/2 lmo1/3 BarH-like barhl1.1 barhl2 lmo1 PTPRD PTPRF ELAVL5 POU3 Gbx Tob1/2 Tob1/2 AP-2 AP-2 ACBD6 Emx HAND Dlx Sp5 MYO3B ELAVL4 Six3 Six2 Tob1 barhl2 barhl2 Id Meis Msx PCNE length 351 491 232 586 40 45 68 554 48 43 42 227 51 45 42 41 45 40 41 48 57 46 46 172 41 45 299 52 64 69 76 391 67 303 55 43 49 42 72 42 56 54 447 477 144 485 175 222 285 451 192 150 239 PCR fragment size 720 578 671 849 209 407 389 945 532 550 416 547 440 433 394 417 413 386 383 416 448 155 372 551 102 643 627 429 430 459 463 468 455 685 437 439 528 442 463 435 441 330 594 672 218 646 363 389 397 639 576 542 586 442 322 431 453 453 315 415 412 285 #SCREENED 363 371 96 179 161 212 76 151 365 434 209 415 154 344 236 137 386 351 106 278 243 145 111 161 211 313 115 168 124 152 86 56 140 172 120 158 138 150 126 97 240 150 258 259 115 343 180 115 298 229 454 187 574 78 218 131 126 224 498 293 189 236 283 #POS 113 146 37 7 1 36 11 64 22 12 8 31 20 5 0 6 14 47 3 1 2 3 12 25 0 51 41 39 33 31 3 11 8 2 82 6 6 3 3 1 10 2 48 12 2 38 2 10 8 7 54 2 160 2 3 5 9 0 4 27 0 0 6 #NEG 250 225 59 172 160 176 65 87 343 422 201 384 134 339 236 131 372 304 103 277 241 142 99 136 211 262 74 129 91 121 83 45 132 170 38 152 132 147 123 96 230 148 210 247 113 305 178 105 290 222 400 185 414 76 215 126 117 224 494 266 189 236 277 #TISSCAT A-E)CNS/PNS 78 126 36 2 0 29 9 55 7 1 5 11 1 0 0 0 3 21 2 0 1 3 11 16 0 48 33 32 33 22 2 3 1 1 7 3 3 1 0 1 0 1 25 5 1 18 1 3 4 4 2 1 16 1 0 5 0 0 1 0 0 0 2 #TISSUE A)forebrain 28 34 28 1 0 5 2 16 0 0 0 4 0 0 0 0 0 2 0 0 0 0 0 0 0 6 9 1 0 0 0 0 0 0 4 0 1 0 0 0 0 0 2 0 1 0 0 0 1 0 0 0 1 0 0 2 0 0 1 0 0 0 0 #TISSSUBDV 1)telencephalon 28 12 27 1 0 1 1 16 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 6 1 0 0 0 0 0 0 4 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 1 0 0 2 0 0 0 0 0 0 0 #TISSSUBDV 2)diencephalon 0 25 3 0 0 5 1 9 0 0 0 4 0 0 0 0 0 2 0 0 0 0 0 0 0 4 4 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 #TISSSUBDV 3)epiphysis 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 #TISSUE B)midbrain 1 53 5 0 0 4 4 9 0 0 0 7 1 0 0 0 0 0 0 0 0 1 0 0 0 0 5 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 1 0 0 1 0 0 1 1 1 0 3 0 0 0 0 0 0 0 #TISSSUBDV 1)tectum 1 21 4 0 0 1 0 8 0 0 0 4 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 #TISSSUBDV 2)tegmentum 0 49 4 0 0 4 4 9 0 0 0 3 0 0 0 0 0 0 0 0 0 1 0 0 0 0 5 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 1 0 0 1 0 0 1 0 0 0 2 0 0 0 0 0 0 0 #TISSSUBDV 3)MHB (midbrain-hindbrain boundary) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 #TISSUE C)hindbrain 0 89 10 0 0 9 5 39 0 1 2 1 0 0 0 0 1 0 1 0 0 0 0 8 0 8 15 14 18 3 0 0 0 0 2 1 2 0 0 0 0 0 11 0 0 7 1 0 1 2 1 0 4 0 0 2 0 0 0 0 0 0 0 #TISSSUBDV 1)cerebellum 0 43 2 0 0 1 2 9 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 #TISSSUBDV 2)rhombencephalon (r1-r7) 0 83 8 0 0 9 3 38 0 1 2 1 0 0 0 0 1 0 1 0 0 0 0 8 0 7 12 13 18 3 0 0 0 0 1 1 2 0 0 0 0 0 11 0 0 7 1 0 1 2 1 0 4 0 0 2 0 0 0 0 0 0 0 #TISSUE D)spinal cord 65 59 4 1 0 18 1 31 7 0 3 0 0 0 0 0 2 19 1 0 1 2 11 9 0 35 13 18 17 19 2 3 1 1 1 2 1 1 0 1 0 1 9 5 0 10 0 3 1 2 2 0 10 0 0 0 0 0 0 0 0 0 2 #TISSUE E)other neuronal cells 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 #TISSCAT F-H)Sensory Organs 0 12 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 #TISSUE F)eye 0 11 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 #TISSSUBDV 1)retina 0 11 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 #TISSSUBDV 2)lens 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 #TISSUE G)ear/otic vesicle 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 #TISSUE H)lateral line (neuromasts and/or primordium) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 #TISSUE I)Notochord 7 3 0 2 0 0 0 13 0 0 0 6 0 1 0 0 3 1 0 0 0 0 0 13 0 0 0 2 0 13 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 81 0 3 0 9 0 0 0 0 0 0 #TISSUE J)Muscle 65 13 0 1 0 0 0 2 2 2 0 9 3 0 0 4 3 16 0 0 1 0 0 0 0 0 13 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 3 0 7 0 0 45 0 38 0 0 0 0 0 0 27 0 0 0 #TISSUE K)Blood/blood islands 1 3 0 0 0 1 0 0 1 0 1 2 11 1 0 0 1 18 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 0 1 0 1 0 2 1 0 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 #TISSUE L)Heart/pericardium 0 13 0 2 0 6 1 3 1 0 0 6 0 1 0 0 5 6 0 0 0 0 0 0 0 3 0 3 1 2 1 3 3 0 1 1 0 1 1 0 5 1 14 2 0 4 1 0 2 0 4 0 0 0 0 0 0 0 0 0 0 0 0 #TISSCAT M-P)Skin/Fins 0 1 1 0 0 0 0 4 0 0 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 1 0 2 0 0 1 0 4 0 62 0 0 0 0 0 0 0 0 0 0 #TISSUE M)Epidermis/EVL (including pigment cells) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 #TISSCAT O-P)Fin 0 1 1 0 0 0 0 4 0 0 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 1 0 2 0 0 1 0 4 0 62 0 0 0 0 0 0 0 0 0 0 #TISSUE O)Fin fold (dorsal/median/tail) 0 1 1 0 0 0 0 4 0 0 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 1 0 2 0 0 1 0 4 0 62 0 0 0 0 0 0 0 0 0 0 #TISSUE P) Pectoral Fin 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 #TISSCAT Q-V)Other 53 8 1 0 1 5 2 5 12 9 3 9 16 3 0 3 2 7 1 1 0 0 2 0 0 0 1 2 1 0 0 4 3 0 78 1 2 3 1 0 6 1 20 6 1 8 0 0 2 5 2 1 13 0 0 0 0 0 3 0 0 0 5 #TISSUE Q)Tailbud 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 #TISSUE R)Yolk/YSL/Hatching Gland 50 4 0 0 1 3 1 5 12 9 0 5 9 2 0 2 0 7 0 1 0 0 1 0 0 0 1 2 1 0 0 4 3 0 78 1 2 2 1 0 5 1 16 6 1 8 0 0 2 4 1 1 0 0 0 0 0 0 0 0 0 0 0 #TISSUE S)Between brain (fb/mb/hb) and yolk 1 0 0 0 0 0 1 0 0 0 0 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 #TISSUE T)Between spinal cord and yolk/yolk extension 2 4 1 0 0 2 0 0 0 0 3 2 10 1 0 1 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 3 0 0 0 0 0 0 1 1 0 9 0 0 0 0 0 3 0 0 0 2 #TISSUE U)Ventral/Caudal (caudal to end of Yolk extension) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 #TISSUE V)Other (unidentified) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PERCENT 0.31 0.39 0.39 0.04 0.01 0.17 0.14 0.42 0.06 0.03 0.04 0.07 0.13 0.01 0 0.04 0.04 0.13 0.03 0 0.01 0.02 0.11 0.16 0 0.16 0.36 0.23 0.27 0.2 0.03 0.2 0.06 0.01 0.68 0.04 0.04 0.02 0.02 0.01 0.04 0.01 0.19 0.05 0.02 0.11 0.01 0.09 0.03 0.03 0.12 0.01 0.28 0.03 0.01 0.04 0.07 0 0.01 0.09 0 0 0.02