TY  - JOUR
A1  - Suter, Bernhard
A1  - Fetchko, Michael J.
A1  - Imhof, Ralph
A1  - Graham, Christopher I.
A1  - Stoffel-Studer, Ingrid
A1  - Zbinden, Caroline
A1  - Raghavan, Maanasa
A1  - Lopez, Lianet
A1  - Beneti, Lucija
A1  - Hort, Jacqueline
A1  - Fillingham, Jeffrey
A1  - Greenblatt, Jack F.
A1  - Giaever, Guri
A1  - Nislow, Corey
A1  - Stagljar, Igor
T1  - Examining protein–protein interactions using endogenously tagged yeast arrays: The Cross-and-Capture system
Y1  - 2007/12/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 000 
EP  - 000 
DO  - 10.1101/gr.6667007 
VL  - 17 
IS  - 12 
UR  - http://genome.cshlp.org/content/early/2007/11/07/gr.6667007.abstract 
N2  - Comprehensive approaches to detect protein–protein interactions (PPIs) have been most successful in the yeast model system. Here we present “Cross-and-Capture,” a novel assay for rapid, sensitive assessment of PPIs via pulldown of differently tagged yeast strain arrays. About 500 yeast genes that function in DNA replication, repair, and recombination and nuclear proteins of unknown function were chromosomally tagged with six histidine residues or triple VSV epitopes. We demonstrate that the assay can interrogate a wide range of previously known protein complexes with increased resolution and sensitivity. Furthermore, we use “Cross-and-Capture” to identify two novel protein complexes: Rtt101p–Mms1p and Sae2p–Mre11p. The Rtt101p–Mms1p interaction was subsequently characterized by genetic and functional analyses. Our studies establish the “Cross-and-Capture” assay as a novel, versatile tool that provides a valuable complement for the next generation of yeast proteomic studies. 
ER  -