RT Journal
A1 Jeong, Sangkyun
A1 Hahn, Yoonsoo
A1 Rong, Qi
A1 Pfeifer, Karl
T1 Accurate quantitation of allele-specific expression patterns by analysis of DNA melting
JF Genome Research 
JO Genome Research 
YR 2007 
FD June 01 
VO 17 
IS 8 
SP 000 
OP 000 
DO 10.1101/gr.6028507 
UL http://genome.cshlp.org/content/early/2007/06/01/gr.6028507.abstract 
AB Epigenetic and genetic mechanisms can result in large differences in expression levels of the two alleles in a diploid organism. Furthermore, these differences may be critical to phenotypic variations among individuals. In this study, we present a novel procedure and algorithm to precisely and accurately quantitate the relative expression of each allele. This method uses the differential melting properties of DNAs differing at even a single base pair. By referring to the melting characteristics of the two pure alleles, the fractional contribution of the two alleles to any unknown mixture can be mathematically resolved. These methods are highly accurate and precise because each single melting reaction yields multiple data points for analysis. Finally, we discuss how this approach can be used more generally to accurately quantitate gene expression relative to known standards.