RT Journal
A1 Jehan, Zeenath
A1 Vallinayagam, Sambandam
A1 Tiwari, Shrish
A1 Pradhan, Suman
A1 Singh, Lalji
A1 Suresh, Amritha
A1 Reddy, Hemakumar M.
A1 Ahuja, Y.R.
A1 Jesudasan, Rachel A.
T1 Novel noncoding RNA from human Y distal heterochromatic block (Yq12) generates testis-specific chimeric CDC2L2
JF Genome Research 
JO Genome Research 
YR 2006 
FD December 01 
VO 16 
IS 12 
SP 000 
OP 000 
DO 10.1101/gr.5155706 
UL http://genome.cshlp.org/content/early/2006/11/09/gr.5155706.abstract 
AB The human Y chromosome, because it is enriched in repetitive DNA, has been very intractable to genetic and molecular analyses. There is no previous evidence for developmental stage- and testis-specific transcription from the male-specific region of the Y (MSY). Here, we present evidence for the first time for a developmental stage- and testis-specific transcription from MSY distal heterochromatic block. We isolated two novel RNAs, which localize to Yq12 in multiple copies, show testis-specific expression, and lack active X-homologs. Experimental evidence shows that one of the above Yq12 noncoding RNAs (ncRNAs) trans-splices with CDC2L2 mRNA from chromosome 1p36.3 locus to generate a testis-specific chimeric β sv13 isoform. This 67-nt 5′UTR provided by the Yq12 transcript contains within it a Y box protein-binding CCAAT motif, indicating translational regulation of the β sv13 isoform in testis. This is also the first report of trans-splicing between a Y chromosomal and an autosomal transcript.