TY  - JOUR
A1  - Zhang, Yali
A1  - Zhou, Yayi
A1  - Chen, Yangyang
A1  - Wang, Xueyu
A1  - Hu, Boyan
A1  - Siddique, Shahid
A1  - Latina, Romnick A.
A1  - Casey, Veronica I.
A1  - Bo, Dexin
A1  - Liao, Yucheng
A1  - Zhang, Min
A1  - Sun, Ming
A1  - Zhang, Fengjuan
A1  - Dai, Dadong
T1  - Haplotype-resolved telomere-to-telomere genome of Aphelenchus avenae implicates P5CS in nematode desiccation stress response
Y1  - 2026/03/23 
JF  - Genome Research 
JO  - Genome Research 
DO  - 10.1101/gr.281016.125 
UR  - http://genome.cshlp.org/content/early/2026/03/20/gr.281016.125.abstract 
N2  - The remarkable ability to survive desiccation and persist in a dry state is among nature's most fascinating adaptations, enabling certain organisms to withstand extreme dehydration without damage. This phenomenon has been widespread across diverse life forms, including plants, fungi, and nematodes. However, our understanding of its molecular basis, particularly in animals, remains limited. Aphelenchus avenae nematodes are notable for their exceptional tolerance to dehydration, and multiple genes related to this trait have been identified. However, the absence of a chromosome-scale, high-contiguity genome for A. avenae has been a limitation in the genome-wide identification of gene families potentially involved in desiccation tolerance. In this study, we assemble a high-quality, telomere-to-telomere haplotype genome of A. avenae. Transcriptomic analyses reveal distinct sets of genes involved in responses to desiccation and freezing stress. Notably, under desiccation stress, several desiccation-tolerance genes exhibit allelic imbalance expression. Among these, we identify the stress response gene P5CS in A. avenae nematodes. Aap5cs RNAi experiments demonstrate that knockdown of Aap5cs results in increased accumulation of reactive oxygen species under desiccation stress and reduced desiccation survival time, suggesting that Aap5cs plays a role in the stress response of A. avenae. This finding also raises the possibility of functional parallels between plant and nematode responses to dehydration. This study enhances our understanding of nematode resistance to desiccation stress and provides valuable genetic resources for investigating the intricate regulatory pathway that organisms use for dehydration stress adaptation. 
ER  -