RT Journal
A1 Zhao, Jingjing
A1 Baltoumas, Fotis A
A1 Konnaris, Maxwell A
A1 Mouratidis, Ioannis
A1 Liu, Zhe
A1 Sims, Jasmine
A1 Agarwal, Vikram
A1 Pavlopoulos, Georgios A
A1 Georgakopoulos-Soares, Ilias
A1 Ahituv, Nadav
T1 MPRAbase a Massively Parallel Reporter Assay database
JF Genome Research 
JO Genome Research 
YR 2025 
FD April 22 
DO 10.1101/gr.280387.124 
SP gr.280387.124 
UL http://genome.cshlp.org/content/early/2025/04/22/gr.280387.124.abstract 
AB Massively parallel reporter assays (MPRAs) represent a set of high-throughput technologies that measure the functional effects of thousands of sequences/variants on gene regulatory activity. There are several different variations of MPRA technology and they are used for numerous applications, including regulatory element discovery, variant effect measurement, saturation mutagenesis, synthetic regulatory element generation or characterization of evolutionary gene regulatory differences. Despite their many designs and uses, there is no comprehensive database that incorporates the results of these experiments. To address this, we developed MPRAbase, a manually curated database that currently harbors 130 experiments, encompassing 17,718,677 elements tested across 35 cell types and 4 organisms. The MPRAbase web interface serves as a centralized user-friendly repository to examine online the activity of regulatory elements across cell types and organisms, and to download MPRA data for independent analysis.