RT Journal
A1 Huang, Ying
A1 Sircar, Sanchari
A1 Ramirez-Prado, Juan Sebastian
A1 Manza-Mianza, Deborah
A1 Antunez-Sanchez, Javier
A1 brik-chaouche, Rim
A1 Rodriguez-Granados, Natalia
A1 An, Jing
A1 Bergounioux, Catherine
A1 Mahfouz, Magdy
A1 Hirt, Heribert
A1 Crespi, Martin
A1 Concia, Lorenzo
A1 Barnech, Fredy
A1 Amiard, Simon
A1 Probst, Aline V
A1 Gutierrez-Marcos, Jose
A1 Ariel, Federico
A1 Raynaud, Cecile
A1 Latrasse, David
A1 Benhamed, Moussa
T1 Polycomb-dependent differential chromatin compartmentalization determines gene coregulation in Arabidopsis
JF Genome Research 
JO Genome Research 
YR 2021 
FD June 03 
DO 10.1101/gr.273771.120 
SP gr.273771.120 
UL http://genome.cshlp.org/content/early/2021/06/03/gr.273771.120.abstract 
AB In animals, distant H3K27me3-marked Polycomb targets can establish physical interactions forming repressive chromatin hubs. In plants, growing evidence suggests that H3K27me3 act directly or indirectly to regulate chromatin interactions, although how this histone modification modulates 3D chromatin architecture remains elusive. To decipher the impact of the dynamic deposition of H3K27me3 on the Arabidopsis thaliana nuclear interactome, we combined genetics, transcriptomics and alternative 3D epigenomic approaches. By analyzing mutants defective for histone H3K27 methylation or demethylation we uncovered the crucial role of this chromatin mark in short- and previously unnoticed long-range chromatin loop formation. We found that a reduction in H3K27me3 led to a decrease in the interactions within Polycomb-associated repressive domains. Regions with lower H3K27me3 levels in the H3K27 methyltransferase clf mutant established new interactions with regions marked with H3K9ac – a histone modification associated with active transcription, thus indicating that a reduction in H3K27me3 levels induces a global reconfiguration of chromatin architecture. Altogether, our results reveal that the 3D genome organization is tightly linked to reversible histone modifications that govern chromatin interactions. Consequently, nuclear organization dynamics shapes the transcriptional reprogramming during plant development and places H3K27me3 as a key feature in the coregulation of distant genes.