TY  - JOUR
A1  - Huang, Ying
A1  - Sircar, Sanchari
A1  - Ramirez-Prado, Juan Sebastian
A1  - Manza-Mianza, Deborah
A1  - Antunez-Sanchez, Javier
A1  - brik-chaouche, Rim
A1  - Rodriguez-Granados, Natalia
A1  - An, Jing
A1  - Bergounioux, Catherine
A1  - Mahfouz, Magdy
A1  - Hirt, Heribert
A1  - Crespi, Martin
A1  - Concia, Lorenzo
A1  - Barnech, Fredy
A1  - Amiard, Simon
A1  - Probst, Aline V
A1  - Gutierrez-Marcos, Jose
A1  - Ariel, Federico
A1  - Raynaud, Cecile
A1  - Latrasse, David
A1  - Benhamed, Moussa
T1  - Polycomb-dependent differential chromatin compartmentalization determines gene coregulation in Arabidopsis
Y1  - 2021/06/03 
JF  - Genome Research 
JO  - Genome Research 
DO  - 10.1101/gr.273771.120 
SP  - gr.273771.120 
UR  - http://genome.cshlp.org/content/early/2021/06/03/gr.273771.120.abstract 
N2  - In animals, distant H3K27me3-marked Polycomb targets can establish physical interactions forming repressive chromatin hubs. In plants, growing evidence suggests that H3K27me3 act directly or indirectly to regulate chromatin interactions, although how this histone modification modulates 3D chromatin architecture remains elusive. To decipher the impact of the dynamic deposition of H3K27me3 on the Arabidopsis thaliana nuclear interactome, we combined genetics, transcriptomics and alternative 3D epigenomic approaches. By analyzing mutants defective for histone H3K27 methylation or demethylation we uncovered the crucial role of this chromatin mark in short- and previously unnoticed long-range chromatin loop formation. We found that a reduction in H3K27me3 led to a decrease in the interactions within Polycomb-associated repressive domains. Regions with lower H3K27me3 levels in the H3K27 methyltransferase clf mutant established new interactions with regions marked with H3K9ac – a histone modification associated with active transcription, thus indicating that a reduction in H3K27me3 levels induces a global reconfiguration of chromatin architecture. Altogether, our results reveal that the 3D genome organization is tightly linked to reversible histone modifications that govern chromatin interactions. Consequently, nuclear organization dynamics shapes the transcriptional reprogramming during plant development and places H3K27me3 as a key feature in the coregulation of distant genes. 
ER  -