RT Journal
A1 Lin, Juncheng
A1 Hung, Fu-Yu
A1 Ye, Congting
A1 Hong, Liwei
A1 Shih, Yuan-Hsin
A1 Wu, Keqiang
A1 Li, Qingshun Quinn
T1 HDA6 dependent chromatin deacetylation orchestrates mRNA polyadenylation
JF Genome Research 
JO Genome Research 
YR 2020 
FD August 05 
DO 10.1101/gr.255232.119 
SP gr.255232.119 
UL http://genome.cshlp.org/content/early/2020/08/04/gr.255232.119.abstract 
AB Eukaryotic histone deacetylation, critical for maintaining nucleosome structure and regulating gene expression, is mediated by histone deacetylases (HDACs). While nucleosomes have been reported to regulate mRNA polyadenylation in humans, the role of HDACs in regulating polyadenylation has not been uncovered. Taking the advantage of phenotypic studies on Arabidopsis, HDA6 (one of HDACs) was found to be a critical part of many biological processes. Here, we report that HDA6 affects mRNA polyadenylation of Arabidopsis. Poly(A) sites of upregulated transcripts are closer to the histone acetylation peaks in hda6 compared to the wild-type Col-0. HDA6 is required for the deacetylation of histones around DNA on nucleosomes, which solely coincides with up-regulated or uniquely presented poly(A) sites in hda6. Furthermore, defective HDA6 results in an over-representation of the canonical poly(A) signal (AAUAAA) usages. Chromatins for generating AAUAAA-type transcripts have a comparatively low H3K9K14ac around poly(A) sites when compared to other non-canonical poly(A) signal containing transcripts. These results indicate that HDA6 regulates polyadenylation in a histone deacetylation dependent manner in Arabidopsis.