TY  - JOUR
A1  - Yoshimura, Jun
A1  - Ichikawa, Kazuki
A1  - Shoura, Massa J.
A1  - Artiles, Karen L.
A1  - Gabdank, Idan
A1  - Wahba, Lamia
A1  - Smith, Cheryl L.
A1  - Edgley, Mark L.
A1  - Rougvie, Ann E.
A1  - Fire, Andrew Z.
A1  - Morishita, Shinichi
A1  - Schwarz, Erich M.
T1  - Recompleting the Caenorhabditis elegans genome
Y1  - 2019/05/23 
JF  - Genome Research 
JO  - Genome Research 
DO  - 10.1101/gr.244830.118 
UR  - http://genome.cshlp.org/content/early/2019/05/20/gr.244830.118.abstract 
N2  - Caenorhabditis elegans was the first multicellular eukaryotic genome sequenced to apparent completion. Although this assembly employed a standard C. elegans strain (N2), it used sequence data from several laboratories, with DNA propagated in bacteria and yeast. Thus, the N2 assembly has many differences from any C. elegans available today. To provide a more accurate C. elegans genome, we performed long-read assembly of VC2010, a modern strain derived from N2. Our VC2010 assembly has 99.98% identity to N2 but with an additional 1.8 Mb including tandem repeat expansions and genome duplications. For 116 structural discrepancies between N2 and VC2010, 97 structures matching VC2010 (84%) were also found in two outgroup strains, implying deficiencies in N2. Over 98% of N2 genes encoded unchanged products in VC2010; moreover, we predicted ≥53 new genes in VC2010. The recompleted genome of C. elegans should be a valuable resource for genetics, genomics, and systems biology. 
ER  -