TY  - JOUR
A1  - Carlevaro-Fita, Joana
A1  - Polidori, Taisia
A1  - Das, Monalisa
A1  - Navarro, Carmen
A1  - Zoller, Tatjana Irina
A1  - Johnson, Rory
T1  - Ancient exapted transposable elements promote nuclear enrichment of human long noncoding RNAs
Y1  - 2018/12/26 
JF  - Genome Research 
JO  - Genome Research 
DO  - 10.1101/gr.229922.117 
SP  - gr.229922.117 
UR  - http://genome.cshlp.org/content/early/2018/12/21/gr.229922.117.abstract 
N2  -  The sequence domains underlying long noncoding RNA (lncRNA) activities, including their characteristic nuclear enrichment, remain largely unknown. It has been proposed that these domains can originate from neofunctionalised fragments of transposable elements (TEs), otherwise known as RIDLs (Repeat Insertion Domains of Long Noncoding RNA), although just a handful have been identified. It is challenging to distinguish functional RIDL instances against a numerous genomic background of neutrally-evolving TEs. We show evidence that a subset of TE types experience evolutionary selection in the context of lncRNA exons. Together these comprise an enrichment group of 5374 TE fragments in 3566 loci. Their host lncRNAs tend to be functionally validated and associated with disease. This RIDL group was used to explore the relationship between TEs and lncRNA subcellular localisation. Using global localisation data from ten human cell lines, we uncover a dose-dependent relationship between nuclear/cytoplasmic distribution, and evolutionarily-conserved L2b, MIRb and MIRc elements. This is observed in multiple cell types, and is unaffected by confounders of transcript length or expression. Experimental validation with engineered transgenes shows that these TEs drive nuclear enrichment in a natural sequence context. Together these data reveal a role for TEs in regulating the subcellular localisation of lncRNAs. 
ER  -