TY  - JOUR
A1  - Gao, Yan
A1  - Ni, Xiaohui
A1  - Guo, Hua
A1  - Su, Zhe
A1  - Ba, Yi
A1  - Tong, Zhongsheng
A1  - Guo, Zhi
A1  - Yao, Xin
A1  - Chen, Xixi
A1  - Yin, Jian
A1  - Yan, Zhao
A1  - Guo, Lin
A1  - Liu, Ying
A1  - Bai, Fan
A1  - Xie, Xiaoliang Sunney
A1  - Zhang, Ning
T1  - Single-cell sequencing deciphers a convergent evolution of copy number alterations from primary to circulating tumour cells
Y1  - 2017/05/09 
JF  - Genome Research 
JO  - Genome Research 
DO  - 10.1101/gr.216788.116 
SP  - gr.216788.116 
UR  - http://genome.cshlp.org/content/early/2017/05/08/gr.216788.116.abstract 
N2  - Copy number alteration (CNA) is a major contributor to genome instability, a hallmark of cancer. Here we studied genomic alterations in single primary tumour cells and circulating tumour cells (CTCs) from the same patient. Single-nucleotide variations (SNVs) in single cells from both samples occurred sporadically, whereas CNAs among primary tumour cells emerged accumulatively rather than abruptly, converging toward that of CTCs. Focal CNAs affecting MYC gene and PTEN gene were observed only in a minor portion of primary tumour cells but were present in all CTCs, suggesting a strong selection toward metastasis. Single-cell structural variation (SV) analyses revealed a two-step mechanism, a complex rearrangement followed by gene amplification, for the simultaneous formation of anomalous CNAs in multiple chromosome regions. Integrative CNA analyses of 97 CTCs from 23 patients confirmed the convergence of CNAs and revealed single, concurrent, and mutually exclusive CNAs that could be the driving events in cancer metastasis. 
ER  -