RT Journal
A1 Kajitani, Rei
A1 Toshimoto, Kouta
A1 Noguchi, Hideki
A1 Toyoda, Atsushi
A1 Ogura, Yoshitoshi
A1 Okuno, Miki
A1 Yabana, Mitsuru
A1 Harada, Masahira
A1 Nagayasu, Eiji
A1 Maruyama, Haruhiko
A1 Kohara, Yuji
A1 Fujiyama, Asao
A1 Hayashi, Tetsuya
A1 Itoh, Takehiko
T1 Efficient de novo assembly of highly heterozygous genomes from whole-genome shotgun short reads
JF Genome Research 
JO Genome Research 
YR 2014 
FD April 22 
DO 10.1101/gr.170720.113 
SP gr.170720.113 
UL http://genome.cshlp.org/content/early/2014/04/22/gr.170720.113.abstract 
AB Although many de novo genome assembly projects have recently been performed using high-throughput sequencers, assembling the highly heterozygous diploid genomes is a big scientific challenge due to the increased complexity of the de Bruijn graph structure predominantly employed. To deal with an increasing demand for sequencing of non-model and/or wild-type sample, in most cases, inbred lines or fosmid-based hierarchical sequencing methods are employed with overcoming such problems. However, these methods are costly and time consuming, forfeiting the advantage of massive parallel sequencing. Here, we describe a novel de novo assembler, Platanus, which can effectively manage high-throughput data from heterozygous samples. Platanus assembles DNA fragments (reads) into contigs by constructing de Bruijn graphs with automatically optimized k-mer sizes, followed by scaffolding of contigs based on paired-end information. The complicated graph structures that result from the heterozygosity are simplified during not only the contig assembly step but also the scaffolding step. We evaluated the assembly results on eukaryotic samples with various levels of heterozygosity. Compared with other assemblers, the Platanus assembly results have a larger NG50 length without any accompanying loss of accuracy in both simulated data and real data. In addition, Platanus recorded the largest NG50 values for two of the three low heterozygous species used in the de novo assembly contest, Assemblathon2. Platanus provides, therefore, a novel and efficient approach for the assembly of Giga base-sized highly heterozygous genomes and is also an attractive alternative to the existing assemblers designed for genomes of lower heterozygosity.