RT Journal
A1 Akagi, Keiko
A1 Li, Jingfeng
A1 Broutian, Tatevik R
A1 Padilla-Nash, Hesed
A1 Xiao, Weihong
A1 Jiang, Bo
A1 Rocco, James W
A1 Teknos, Theodoros N
A1 Kumar, Bhavna
A1 Wangsa, Danny
A1 He, Dandan
A1 Ried, Thomas
A1 Symer, David E
A1 Gillison, Maura L
T1 Genome-wide analysis of HPV integration in human cancers reveals recurrent, focal genomic instability
JF Genome Research 
JO Genome Research 
YR 2013 
FD November 07 
DO 10.1101/gr.164806.113 
SP gr.164806.113 
UL http://genome.cshlp.org/content/early/2013/11/05/gr.164806.113.abstract 
AB Genomic instability is a hallmark of human cancers, including the 5% caused by human papillomavirus (HPV). Here we report a striking association between HPV integration and adjacent host genomic structural variation in human cancer cell lines and primary tumors. Whole genome sequencing revealed HPV integrants flanking and bridging extensive host genomic amplifications and rearrangements, including deletions, inversions and chromosomal translocations. We present a model of 'looping' by which HPV integrant-mediated DNA replication and recombination may result in viral-host DNA concatemers, frequently disrupting genes involved in oncogenesis and amplifying HPV oncogenes E6 and E7. Our high-resolution results shed new light on a catastrophic process, distinct from chromothripsis and other mutational processes, by which HPV directly promotes genomic instability.