TY  - JOUR
A1  - Sexton, Brittany S.
A1  - Avey, Denis
A1  - Druliner, Brooke R.
A1  - Fincher, Justin A.
A1  - Vera, Daniel L.
A1  - Grau, Daniel J.
A1  - Borowsky, Mark L.
A1  - Gupta, Shobhit
A1  - Girimurugan, Senthil
A1  - Chicken, Eric
A1  - Zhang, Jinfeng
A1  - Noble, William S.
A1  - Zhu, Fanxiu
A1  - Kingston, Robert E.
A1  - Dennis, Jonathan Hancock
T1  - The spring-loaded genome: Nucleosome redistributions are widespread, transient, and DNA-directed
Y1  - 2013/12/05 
JF  - Genome Research 
JO  - Genome Research 
DO  - 10.1101/gr.160150.113 
SP  - gr.160150.113 
UR  - http://genome.cshlp.org/content/early/2013/12/05/gr.160150.113.abstract 
N2  - Nucleosome occupancy plays a key role in regulating access to the eukaryotic genomes.  Although various chromatin regulatory complexes are known to regulate nucleosome occupancy, the role of DNA sequence in this regulation remains unclear, particularly in mammals.  To address this problem, we measured nucleosome distribution at high temporal resolution in human cells at hundreds of genes during the reactivation of Kaposi's sarcoma-associated herpesvirus (KSHV).  We show that nucleosome redistribution peaks at 24 hours post KSHV reactivation and that the nucleosomal redistributions are widespread and transient. To clarify the role of DNA sequence in these nucleosomal redistributions, we compared the genes with altered nucleosome distribution to a sequence-based computer model and in vitro assembled nucleosomes.  We demonstrate that both the predicted model and the assembly nucleosome distributions are concordant with the majority of nucleosome redistributions at 24 hours post KSHV reactivation.  We suggest a model in which loci are held in unfavorable chromatin architecture and 'spring' to a transient intermediate state directed by DNA sequence information.  We propose that DNA sequence plays a more considerable role in the regulation of nucleosome positions than was previously appreciated.  The surprising findings that nucleosome redistributions are widespread, transient, and DNA-directed shift the current perspective regarding regulation of nucleosome distribution in humans. 
ER  -