TY  - JOUR
A1  - Wu, Jun
A1  - Wang, Zhiwen
A1  - Shi, Zebin
A1  - Zhang, Shu
A1  - Ming, Ray
A1  - Zhu, Shilin
A1  - Khan, M. Awais
A1  - Tao, Shutian
A1  - Korban, Schuyler S.
A1  - Wang, Hao
A1  - Chen, Nancy J.
A1  - Nishio, Takeshi
A1  - Xu, Xun
A1  - Cong, Lin
A1  - Qi, Kaijie
A1  - Huang, Xiaosan
A1  - Wang, Yingtao
A1  - Zhao, Xiang
A1  - Wu, Juyou
A1  - Deng, Cao
A1  - Gou, Caiyun
A1  - Zhou, Weili
A1  - Yin, Hao
A1  - Qin, Gaihua
A1  - Sha, Yuhui
A1  - Tao, Ye
A1  - Chen, Hui
A1  - Yang, Yanan
A1  - Song, Yue
A1  - Zhan, Dongliang
A1  - Wang, Juan
A1  - Li, Leiting
A1  - Dai, Meisong
A1  - Gu, Chao
A1  - Wang, Yuezhi
A1  - Shi, Daihu
A1  - Wang, Xiaowei
A1  - Zhang, Huping
A1  - Zeng, Liang
A1  - Zheng, Danman
A1  - Wang, Chunlei
A1  - Chen, Maoshan
A1  - Wang, Guangbiao
A1  - Xie, Lin
A1  - Sovero, Valpuri
A1  - Sha, Shoufeng
A1  - Huang, Wenjiang
A1  - Zhang, Shujun
A1  - Zhang, Mingyue
A1  - Sun, Jiangmei
A1  - Xu, Linlin
A1  - Li, Yuan
A1  - Liu, Xing
A1  - Li, Qingsong
A1  - Shen, Jiahui
A1  - Wang, Junyi
A1  - Paull, Robert E.
A1  - Bennetzen, Jeffrey L.
A1  - Wang, Jun
A1  - Zhang, Shaoling
T1  - The genome of pear (Pyrus bretschneideri Rehd.)
Y1  - 2012/11/13 
JF  - Genome Research 
JO  - Genome Research 
DO  - 10.1101/gr.144311.112 
SP  - gr.144311.112 
UR  - http://genome.cshlp.org/content/early/2012/11/13/gr.144311.112.abstract 
N2  - The draft genome of pear (Pyrus bretschneideri) using a combination of BAC-by-BAC and next generation sequencing is reported.  A 512.0 Mb sequence corresponding to 97.1% of the estimated genome size of this highly heterozygous species is assembled with 194x coverage.  High-density genetic maps comprising of 2,005 SNP markers anchored 75.5% of the sequence to all 17 chromosomes. The pear genome encodes 42,812 protein-coding genes, and of these, ~28.5% encode multiple isoforms. High quality of the assembly and annotation is assessed and confirmed using Sanger-derived BAC sequences along with transcriptome sequences of different tissues.  Repetitive sequences of 271.9 Mb in length, accounting for 53.1% of the pear genome, are identified.  Simulation of eudicots to the ancestor of Rosaceae has re-constructed nine ancestral chromosomes.  Pear and apple have diverged from each other ~5.4 to 21.5 MYA, and a recent whole-genome duplication (WGD) event must have occurred 30-45 MYA prior to their divergence, but following divergence from strawberry.  When compared with the apple genome sequence, size differences between apple and pear genomes are confirmed mainly due to presence of repetitive sequences predominantly contributed by transposable elements (TEs), while genic regions are similar in both species.  Genes critical for self-incompatibility, lignified stone cells (a unique feature of pear fruit), sorbitol metabolism and volatile compounds of fruit have also been identified.  Multiple candidate SFB genes appear as tandem repeats in the S-locus region of pear; while, lignin synthesis-related gene family expansion and highly expressed gene families of HCT, C3'H, and CCOMT contribute to high accumulation of both G-lignin and S-lignin.  Expansion of S6PDH, SDH, and SOT along with evolutionary relationships of pear and apple have demonstrated their divergence from a common ancestor.  Moreover, α-linolenic acid metabolism is a key pathway for aroma in pear fruit. 
ER  -