TY  - JOUR
A1  - Khil, Pavel P
A1  - Smagulova, Fatima
A1  - Brick, Kevin M
A1  - Camerini-Otero, R. Daniel
A1  - Petukhova, Galina V
T1  - Sensitive mapping of recombination hotspots using sequencing-based detection of ssDNA
Y1  - 2012/02/24 
JF  - Genome Research 
JO  - Genome Research 
DO  - 10.1101/gr.130583.111 
SP  - gr.130583.111 
UR  - http://genome.cshlp.org/content/early/2012/02/24/gr.130583.111.abstract 
N2  - Meiotic DNA double stranded breaks (DSBs) initiate genetic recombination in discrete areas of the genome called recombination hotspots.  DSBs can be directly mapped using chromatin immunoprecipitation followed by sequencing (ChIP-Seq).  Nevertheless, the genome-wide mapping of recombination hotspots in mammals is still a challenge due to the low frequency of recombination, high heterogeneity of the germ cell population and the relatively low efficiency of ChIP. To overcome these limitations we have developed a novel method   single-stranded DNA (ssDNA) sequencing (SSDS)   that specifically detects protein-bound single-stranded DNA at DSB ends.  SSDS comprises a computational framework for the specific detection of ssDNA-derived reads in a sequencing library and a new library preparation procedure for the enrichment of fragments originating from ssDNA.  The use of our technique reduces the non-specific dsDNA background more than ten-fold.  Our method can be extended to other systems where the identification of ssDNA or DSBs is desired. 
ER  -