@article{Khil24022012,
author = {Khil, Pavel P and Smagulova, Fatima and Brick, Kevin M and Camerini-Otero, R. Daniel and Petukhova, Galina V}, 
title = {Sensitive mapping of recombination hotspots using sequencing-based detection of ssDNA},
year = {2012}, 
doi = {10.1101/gr.130583.111}, 
elocation-id = {gr.130583.111}, 
abstract ={Meiotic DNA double stranded breaks (DSBs) initiate genetic recombination in discrete areas of the genome called recombination hotspots.  DSBs can be directly mapped using chromatin immunoprecipitation followed by sequencing (ChIP-Seq).  Nevertheless, the genome-wide mapping of recombination hotspots in mammals is still a challenge due to the low frequency of recombination, high heterogeneity of the germ cell population and the relatively low efficiency of ChIP. To overcome these limitations we have developed a novel method   single-stranded DNA (ssDNA) sequencing (SSDS)   that specifically detects protein-bound single-stranded DNA at DSB ends.  SSDS comprises a computational framework for the specific detection of ssDNA-derived reads in a sequencing library and a new library preparation procedure for the enrichment of fragments originating from ssDNA.  The use of our technique reduces the non-specific dsDNA background more than ten-fold.  Our method can be extended to other systems where the identification of ssDNA or DSBs is desired.}, 
URL = {http://genome.cshlp.org/content/early/2012/02/24/gr.130583.111.abstract}, 
eprint = {http://genome.cshlp.org/content/early/2012/02/24/gr.130583.111.full.pdf+html}, 
journal = {Genome Research} 
}