@article{Fernandez25052011,
author = {Fernandez, Agustin F. and Assenov, Yassen and Martin-Subero, Jose Ignacio and Balint, Balazs and Siebert, Reiner and Taniguchi, Hiroaki and Yamamoto, Hiroyuki and Hidalgo, Manuel and Tan, Aik-Choon and Galm, Oliver and Ferrer, Isidre and Sanchez-Cespedes, Montse and Villanueva, Alberto and Carmona, Javier and Sanchez-Mut, Jose V. and Berdasco, Maria and Moreno, Victor and Capella, Gabriel and Monk, David and Ballestar, Esteban and Ropero, Santiago and Martinez, Ramon and Sanchez-Carbayo, Marta and Prosper, Felipe and Agirre, Xabier and Fraga, Mario F. and Graña, Osvaldo and Perez-Jurado, Luis and Mora, Jaume and Puig, Susana and Prat, Jaime and Badimon, Lina and Puca, Annibale A. and Meltzer, Stephen J. and Lengauer, Thomas and Bridgewater, John and Bock, Christoph and Esteller, Manel}, 
title = {A DNA methylation fingerprint of 1628 human samples},
year = {2011}, 
doi = {10.1101/gr.119867.110}, 
abstract ={Most of the studies characterizing DNA methylation patterns have been restricted to particular genomic loci in a limited number of human samples and pathological conditions. Herein, we present a compromise between an extremely comprehensive study of a human sample population with an intermediate level of resolution of CpGs at the genomic level. We obtained a DNA methylation fingerprint of 1628 human samples in which we interrogated 1505 CpG sites. The DNA methylation patterns revealed show this epigenetic mark to be critical in tissue-type definition and stemness, particularly around transcription start sites that are not within a CpG island. For disease, the generated DNA methylation fingerprints show that, during tumorigenesis, human cancer cells underwent a progressive gain of promoter CpG-island hypermethylation and a loss of CpG methylation in non-CpG-island promoters. Although transformed cells are those in which DNA methylation disruption is more obvious, we observed that other common human diseases, such as neurological and autoimmune disorders, had their own distinct DNA methylation profiles. Most importantly, we provide proof of principle that the DNA methylation fingerprints obtained might be useful for translational purposes by showing that we are able to identify the tumor type origin of cancers of unknown primary origin (CUPs). Thus, the DNA methylation patterns identified across the largest spectrum of samples, tissues, and diseases reported to date constitute a baseline for developing higher-resolution DNA methylation maps and provide important clues concerning the contribution of CpG methylation to tissue identity and its changes in the most prevalent human diseases.}, 
URL = {http://genome.cshlp.org/content/early/2011/07/12/gr.119867.110.abstract}, 
eprint = {http://genome.cshlp.org/content/early/2011/07/12/gr.119867.110.full.pdf+html}, 
journal = {Genome Research} 
}