RT Journal
A1 Li, Ruiqiang
A1 Zhu, Hongmei
A1 Ruan, Jue
A1 Qian, Wubin
A1 Fang, Xiaodong
A1 Shi, Zhongbin
A1 Li, Yingrui
A1 Li, Shengting
A1 Shan, Gao
A1 Kristiansen, Karsten
A1 Li, Songgang
A1 Yang, Huanming
A1 Wang, Jian
A1 Wang, Jun
T1 De novo assembly of human genomes with massively parallel short read sequencing
JF Genome Research 
JO Genome Research 
YR 2009 
FD December 17 
DO 10.1101/gr.097261.109 
UL http://genome.cshlp.org/content/early/2009/12/16/gr.097261.109.abstract 
AB Next-generation massively parallel DNA sequencing technologies provide ultrahigh throughput at a substantially lower unit data cost; however, the data are very short read length sequences, making de novo assembly extremely challenging. Here, we describe a novel method for de novo assembly of large genomes from short read sequences. We successfully assembled both the Asian and African human genome sequences, achieving an N50 contig size of 7.4 and 5.9 kilobases (kb) and scaffold of 446.3 and 61.9 kb, respectively. The development of this de novo short read assembly method creates new opportunities for building reference sequences and carrying out accurate analyses of unexplored genomes in a cost-effective way.