TY  - JOUR
A1  - Li, Ruiqiang
A1  - Zhu, Hongmei
A1  - Ruan, Jue
A1  - Qian, Wubin
A1  - Fang, Xiaodong
A1  - Shi, Zhongbin
A1  - Li, Yingrui
A1  - Li, Shengting
A1  - Shan, Gao
A1  - Kristiansen, Karsten
A1  - Li, Songgang
A1  - Yang, Huanming
A1  - Wang, Jian
A1  - Wang, Jun
T1  - De novo assembly of human genomes with massively parallel short read sequencing
Y1  - 2009/12/17 
JF  - Genome Research 
JO  - Genome Research 
DO  - 10.1101/gr.097261.109 
UR  - http://genome.cshlp.org/content/early/2009/12/16/gr.097261.109.abstract 
N2  - Next-generation massively parallel DNA sequencing technologies provide ultrahigh throughput at a substantially lower unit data cost; however, the data are very short read length sequences, making de novo assembly extremely challenging. Here, we describe a novel method for de novo assembly of large genomes from short read sequences. We successfully assembled both the Asian and African human genome sequences, achieving an N50 contig size of 7.4 and 5.9 kilobases (kb) and scaffold of 446.3 and 61.9 kb, respectively. The development of this de novo short read assembly method creates new opportunities for building reference sequences and carrying out accurate analyses of unexplored genomes in a cost-effective way. 
ER  -