TY  - JOUR
A1  - Liu, Leo X.
A1  - Spoerke, Jill M.
A1  - Mulligan, Evan L.
A1  - Chen, Jing
A1  - Reardon, Brian
A1  - Westlund, Bethany
A1  - Sun, Lin
A1  - Abel, Ken
A1  - Armstrong, Barbara
A1  - Hardiman, Gary
A1  - King, Judith
A1  - McCague, Lisa
A1  - Basson, Michael
A1  - Clover, Ralph
A1  - Johnson, Carl D.
T1  - High-Throughput Isolation of Caenorhabditis elegans Deletion Mutants
Y1  - 1999/09/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 859 
EP  - 867 
DO  - 10.1101/gr.9.9.859 
VL  - 9 
IS  - 9 
UR  - http://genome.cshlp.org/content/9/9/859.abstract 
N2  - The nematode Caenorhabditis elegans is the first animal whose genome is completely sequenced, providing a rich source of gene information relevant to metazoan biology and human disease. This abundant sequence information permits a broad-based gene inactivation approach in C. elegans, in which chemically mutagenized nematode populations are screened by PCR for deletion mutations in a specific targeted gene. By handling mutagenized worm growth, genomic DNA templates, PCR screens, and mutant recovery all in 96-well microtiter plates, we have scaled up this approach to isolate deletion mutations in >100 genes to date. Four chemical mutagens, including ethyl methane sulfonate, ethlynitrosourea, diepoxyoctane, and ultraviolet-activated trimethylpsoralen, induced detectable deletions at comparable frequencies. The deletions averaged ∼1400 bp in size when using a ∼3 kb screening window. The vast majority of detected deletions removed portions of one or more exons, likely resulting in loss of gene function. This approach requires only the knowledge of a target gene sequence and a suitable mutagen, and thus provides a scalable systematic approach to gene inactivation for any organism that can be handled in high density arrays. 
ER  -