RT Journal
A1 Cao, Yicheng
A1 Kang, Hyung Lyun
A1 Xu, Xuequn
A1 Wang, Mei
A1 Dho, So Hee
A1 Huh, Jun Ryul
A1 Lee, Byeong-Jae
A1 Kalush, Francis
A1 Bocskai, Diana
A1 Ding, Yan
A1 Tesmer, Judith G.
A1 Lee, Jonghyeob
A1 Moon, Eunpyo
A1 Jurecic, Vesna
A1 Baldini, Antonio
A1 Weier, Heinz-Ulrich
A1 Doggett, Norman A.
A1 Simon, Melvin I.
A1 Adams, Mark D.
A1 Kim, Ung-Jin
T1 A 12-Mb Complete Coverage BAC Contig Map in Human Chromosome 16p13.1–p11.2
JF Genome Research 
JO Genome Research 
YR 1999 
FD August 01 
VO 9 
IS 8 
SP 763 
OP 774 
DO 10.1101/gr.9.8.763 
UL http://genome.cshlp.org/content/9/8/763.abstract 
AB We have constructed a complete coverage BAC contig map that spans a 12-Mb genomic segment in the human chromosome 16p13.1–p11.2 region. The map consists of 68 previously mapped STSs and 289 BAC clones, 51 of which—corresponding to a total of 7.721 Mb of genomic DNA—have been sequenced, and provides a high resolution physical map of the region. Contigs were initially built based mainly on the analysis of STS contents and restriction fingerprint patterns of the clones. To close the gaps, probes derived from BAC clone ends were used to screen deeper BAC libraries. Clone end sequence data obtained from chromosome 16-specific BACs, as well as from public databases, were used for the identification of BACs that overlap with fully sequenced BACs by means of sequence match. This approach allowed precise alignment of clone overlaps in addition to restriction fingerprint comparison. A freehand contig drawing software tool was developed and used to manage the map data graphically and generate a real scale physical map. The map we present here is ∼3.5 × deep and provides a minimal tiling path that covers the region in an array of contigous, overlapping BACs.