RT Journal
A1 Steen, Robert G.
A1 Kwitek-Black, Anne E.
A1 Glenn, Christopher
A1 Gullings-Handley, Jo
A1 Van Etten, William
A1 Atkinson, O. Scott
A1 Appel, Diane
A1 Twigger, Simon
A1 Muir, Melanie
A1 Mull, Tim
A1 Granados, Mary
A1 Kissebah, Mushira
A1 Russo, Kerri
A1 Crane, Robbin
A1 Popp, Michael
A1 Peden, Marc
A1 Matise, Tara
A1 Brown, Donna M.
A1 Lu, Jian
A1 Kingsmore, Stephen
A1 Tonellato, Peter J.
A1 Rozen, Steve
A1 Slonim, Donna
A1 Young, Peter
A1 Knoblauch, Margit
A1 Provoost, Abraham
A1 Ganten, Detlev
A1 Colman, Steven D.
A1 Rothberg, Jonathan
A1 Lander, Eric S.
A1 Jacob, Howard J.
T1 A High-Density Integrated Genetic Linkage and Radiation Hybrid Map of the Laboratory Rat
JF Genome Research 
JO Genome Research 
YR 1999 
FD June 01 
VO 9 
IS 6 
SP AP1 
OP AP8 
DO 10.1101/gr.9.6.AP1 
UL http://genome.cshlp.org/content/9/6/AP1.abstract 
AB The laboratory rat (Rattus norvegicus) is a key animal model for biomedical research. However, the genetic infrastructure required for connecting phenotype and genotype in the rat is currently incomplete. Here, we report the construction and integration of two genomic maps: a dense genetic linkage map of the rat and the first radiation hybrid (RH) map of the rat. The genetic map was constructed in two F2 intercrosses (SHRSP × BN and FHH × ACI), containing a total of 4736 simple sequence length polymorphism (SSLP) markers. Allele sizes for 4328 of the genetic markers were characterized in 48 of the most commonly used inbred strains. The RH map is a lod ≥ 3 framework map, including 983 SSLPs, thereby allowing integration with markers on various genetic maps and with markers mapped on the RH panel. Together, the maps provide an integrated reference to >3000 genes and ESTs and >8500 genetic markers (5211 of our SSLPs and >3500 SSLPs developed by other groups). [Bihoreau et al. (1997); James and Tanigami, RHdb (http://www.ebi.ac.uk/RHdb/index.html); Wilder (http://www.nih.gov/niams/scientific/ratgbase); Serikawa et al. (1992); RATMAP server (http://ratmap.gen.gu.se)] RH maps (v. 2.0) have been posted on our web sites at http://goliath.ifrc.mcw.edu/LGR/index.htmlor http://curatools.curagen.com/ratmap. Both web sites provide an RH mapping server where investigators can localize their own RH vectors relative to this map. The raw data have been deposited in the RHdb database. Taken together, these maps provide the basic tools for rat genomics. The RH map provides the means to rapidly localize genetic markers, genes, and ESTs within the rat genome. These maps provide the basic tools for rat genomics. They will facilitate studies of multifactorial disease and functional genomics, allow construction of physical maps, and provide a scaffold for both directed and large-scale sequencing efforts and comparative genomics in this important experimental organism.