RT Journal
A1 Chen, Xiangning
A1 Levine, Leanna
A1 Kwok, Pui-Yan
T1 Fluorescence Polarization in Homogeneous Nucleic Acid Analysis
JF Genome Research 
JO Genome Research 
YR 1999 
FD May 01 
VO 9 
IS 5 
SP 492 
OP 498 
DO 10.1101/gr.9.5.492 
UL http://genome.cshlp.org/content/9/5/492.abstract 
AB A new method for DNA diagnostics based on template-directed primer extension and detection by fluorescence polarization is described. In this method, amplified genomic DNA containing a polymorphic locus is incubated with oligonucleotide primers (designed to hybridize to the DNA template adjacent to the polymorphic site) in the presence of allele-specific dye-labeled dideoxyribonucleoside triphosphates and a commercially available modified Taq DNA polymerase. The primer is extended by the dye-terminator specific for the allele present on the template, increasing ∼10-fold the molecular weight of the fluorophore. At the end of the reaction, the fluorescence polarization of the two dye-terminators in the reaction mixture are analyzed directly without separation or purification. This homogeneous DNA diagnostic method is shown to be highly sensitive and specific and is suitable for automated genotyping of large number of samples.[The data shown in Figure 3 are available as an online supplement at http://www.genome.org.]