RT Journal
A1 Marziali, Andre
A1 Willis, Thomas D.
A1 Federspiel, Nancy A.
A1 Davis, Ronald W.
T1 An Automated Sample Preparation System for Large-Scale DNA Sequencing
JF Genome Research 
JO Genome Research 
YR 1999 
FD May 01 
VO 9 
IS 5 
SP 457 
OP 462 
DO 10.1101/gr.9.5.457 
UL http://genome.cshlp.org/content/9/5/457.abstract 
AB Recent advances in DNA sequencing technologies, both in the form of high lane-density gels and automated capillary systems, will lead to an increased requirement for sample preparation systems that operate at low cost and high throughput. As part of the development of a fully automated sequencing system, we have developed an automated subsystem capable of producing 10,000 sequence-ready ssDNA templates per day from libraries of M13 plaques at a cost of $0.29 per sample. This Front End has been in high throughput operation since June, 1997 and has produced > 400,000 high-quality DNA templates.