RT Journal
A1 Gates, Michael A.
A1 Kim, Lisa
A1 Egan, Elizabeth S.
A1 Cardozo, Timothy
A1 Sirotkin, Howard I.
A1 Dougan, Scott T.
A1 Lashkari, Deval
A1 Abagyan, Ruben
A1 Schier, Alexander F.
A1 Talbot, William S.
T1 A Genetic Linkage Map for Zebrafish: Comparative Analysis and Localization of Genes and Expressed Sequences
JF Genome Research 
JO Genome Research 
YR 1999 
FD April 01 
VO 9 
IS 4 
SP 334 
OP 347 
DO 10.1101/gr.9.4.334 
UL http://genome.cshlp.org/content/9/4/334.abstract 
AB Genetic screens in zebrafish (Danio rerio) have isolated mutations in hundreds of genes with essential functions. To facilitate the identification of candidate genes for these mutations, we have genetically mapped 104 genes and expressed sequence tags by scoring single-strand conformational polymorphisms in a panel of haploid siblings. To integrate this map with existing genetic maps, we also scored 275 previously mapped genes, microsatellites, and sequence-tagged sites in the same haploid panel. Systematic phylogenetic analysis defined likely mammalian orthologs of mapped zebrafish genes, and comparison of map positions in zebrafish and mammals identified significant conservation of synteny. This comparative analysis also identified pairs of zebrafish genes that appear to be orthologous to single mammalian genes, suggesting that these genes arose in a genome duplication that occurred in the teleost lineage after the divergence of fish and mammal ancestors. This comparative map analysis will be useful in predicting the locations of zebrafish genes from mammalian gene maps and in understanding the evolution of the vertebrate genome.