TY  - JOUR
A1  - Watson, J.E. Vivienne
A1  - Gabra, Hani
A1  - Taylor, Karen J.
A1  - Rabiasz, Genevieve J.
A1  - Morrison, Harris
A1  - Perry, Paul
A1  - Smyth, John F.
A1  - Porteous, David J.
T1  - Identification and Characterization of a Homozygous Deletion Found in Ovarian Ascites by Representational Difference Analysis
Y1  - 1999/03/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 226 
EP  - 233 
DO  - 10.1101/gr.9.3.226 
VL  - 9 
IS  - 3 
UR  - http://genome.cshlp.org/content/9/3/226.abstract 
N2  - We have performed representational difference analysis (RDA) on DNA from tumor cells and normal fibroblasts isolated from the ascites of a patient with ovarian cancer. Five of six products of the RDA were homozygously deleted from the tumor DNA. One of these products has been characterized and identifies a homozygous deletion of ∼6.9 Mb at chromosome 9p21 in the original ovarian tumor material. This deletion encompasses CDKN2A (p16), CDKN2B (p15), and IFN-α. PCR analysis of other tumor cell lines using the novel STS based on the RDA product has shown it to lie between IFN-α and p16, and to identify the distal extent of a homozygous deletion in another ovarian cancer cell line. These data provide further evidence for a tumor suppressor locus distinct from, but mapping close to, p16 on 9p21. Cytogenetic analysis using comparative genomic hybridization (CGH) performed on the same primary tumor confirmed a loss of material from chromosome 9p. However, the CGH technique had neither the resolution nor the sensitivity to define a subregion of homozygous loss.[The GenBank accession no. for this sequence is AF113912.] 
ER  -