@article{Watson01031999,
author = {Watson, J.E. Vivienne and Gabra, Hani and Taylor, Karen J. and Rabiasz, Genevieve J. and Morrison, Harris and Perry, Paul and Smyth, John F. and Porteous, David J.}, 
title = {Identification and Characterization of a Homozygous Deletion Found in Ovarian Ascites by Representational Difference Analysis},
volume = {9}, 
number = {3}, 
pages = {226-233}, 
year = {1999}, 
doi = {10.1101/gr.9.3.226}, 
abstract ={We have performed representational difference analysis (RDA) on DNA from tumor cells and normal fibroblasts isolated from the ascites of a patient with ovarian cancer. Five of six products of the RDA were homozygously deleted from the tumor DNA. One of these products has been characterized and identifies a homozygous deletion of ∼6.9 Mb at chromosome 9p21 in the original ovarian tumor material. This deletion encompasses CDKN2A (p16), CDKN2B (p15), and IFN-α. PCR analysis of other tumor cell lines using the novel STS based on the RDA product has shown it to lie between IFN-α and p16, and to identify the distal extent of a homozygous deletion in another ovarian cancer cell line. These data provide further evidence for a tumor suppressor locus distinct from, but mapping close to, p16 on 9p21. Cytogenetic analysis using comparative genomic hybridization (CGH) performed on the same primary tumor confirmed a loss of material from chromosome 9p. However, the CGH technique had neither the resolution nor the sensitivity to define a subregion of homozygous loss.[The GenBank accession no. for this sequence is AF113912.]}, 
URL = {http://genome.cshlp.org/content/9/3/226.abstract}, 
eprint = {http://genome.cshlp.org/content/9/3/226.full.pdf+html}, 
journal = {Genome Research} 
}