RT Journal
A1 Piétu, Geneviève
A1 Mariage-Samson, Régine
A1 Fayein, Nicole-Adeline
A1 Matingou, Christiane
A1 Eveno, Eric
A1 Houlgatte, Rémi
A1 Decraene, Charles
A1 Vandenbrouck, Yves
A1 Tahi, Fariza
A1 Devignes, Marie-Dominique
A1 Wirkner, Ute
A1 Ansorge, Wilhelm
A1 Cox, David
A1 Nagase, Takahiro
A1 Nomura, Nobuo
A1 Auffray, Charles
T1 The Genexpress IMAGE Knowledge Base of the Human Brain Transcriptome: A Prototype Integrated Resource for Functional and Computational Genomics
JF Genome Research 
JO Genome Research 
YR 1999 
FD February 01 
VO 9 
IS 2 
SP 195 
OP 209 
DO 10.1101/gr.9.2.195 
UL http://genome.cshlp.org/content/9/2/195.abstract 
AB Expression profiles of 5058 human gene transcripts represented by an array of 7451 clones from the first IMAGE Consortium cDNA library from infant brain have been collected by semiquantitative hybridization of the array with complex probes derived by reverse transcription of mRNA from brain and five other human tissues. Twenty-one percent of the clones corresponded to transcripts that could be classified in general categories of low, moderate, or high abundance. These expression profiles were integrated with cDNA clone and sequence clustering and gene mapping information from an upgraded version of the Genexpress Index. For seven gene transcripts found to be transcribed preferentially or specifically in brain, the expression profiles were confirmed by Northern blot analyses of mRNA from eight adult and four fetal tissues, and 15 distinct regions of brain. In four instances, further documentation of the sites of expression was obtained by in situ hybridization of rat-brain tissue sections. A systematic effort was undertaken to further integrate available cytogenetic, genetic, physical, and genic map informations through radiation-hybrid mapping to provide a unique validated map location for each of these genes in relation to the disease map. The resulting Genexpress IMAGE Knowledge Base is illustrated by five examples presented in the printed article with additional data available on a dedicated Web site at the addresshttp://idefix.upr420.vjf.cnrs.fr/EXPR/welcome.html.