RT Journal
A1 Ohtsuka, Masato
A1 Makino, Satoshi
A1 Yoda, Kinya
A1 Wada, Hironori
A1 Naruse, Kiyoshi
A1 Mitani, Hiroshi
A1 Shima, Akihiro
A1 Ozato, Kenjiro
A1 Kimura, Minoru
A1 Inoko, Hidetoshi
T1 Construction of a Linkage Map of the Medaka (Oryzias latipes) and Mapping of the Da Mutant Locus Defective in Dorsoventral Patterning
JF Genome Research 
JO Genome Research 
YR 1999 
FD December 01 
VO 9 
IS 12 
SP 1277 
OP 1287 
DO 10.1101/gr.9.12.1277 
UL http://genome.cshlp.org/content/9/12/1277.abstract 
AB 
Double anal fin (Da) is a medaka with an autosomal semidominant mutation that causes mirror image duplication of the ventral region concentrating on the caudal region. The chromosomal location of the Da gene and its sequence have remained unknown. We constructed a medaka linkage map as a first step to approach positional cloning of the gene. The segregation analysis was performed on the basis of genetic recombination during female meiosis using 134 random amplified polymorphic DNA (RAPD) markers, 13 sequence-tagged sites (STSs), 15 polymorphic sequences from known genes, and the Da gene. One hundred forty-six markers from the above markers segregated into 26 linkage groups. The size of the genome was estimated to be 1776 cM in length. We identified four syntenic regions between medaka and zebrafish (and human) by mapping the known genes and found one of them to be located in close proximity to theDa gene. By mapping the region surrounding the Dagene in high resolution, two markers were detected flanking theDa gene at 0.32 and 0.80 cM. The detected markers providing a vital clue to initiate chromosome walking will lead us to the definite location of the Da gene.