RT Journal
A1 Wang, Sheng-Yue
A1 Cruts, Marc
A1 Del-Favero, Jurgen
A1 Zhang, Yi
A1 Tissir, Fadel
A1 Potier, Marie-Claude
A1 Patterson, David
A1 Nizetic, Dean
A1 Bosch, Assumpció
A1 Chen, Haiming
A1 Bennett, Lynda
A1 Estivill, Xavier
A1 Kessling, Anna
A1 Antonarakis, Stylianos E.
A1 van Broeckhoven, Christine
T1 A High-Resolution Physical Map of Human Chromosome 21p Using Yeast Artificial Chromosomes
JF Genome Research 
JO Genome Research 
YR 1999 
FD November 01 
VO 9 
IS 11 
SP 1059 
OP 1073 
DO 10.1101/gr.9.11.1059 
UL http://genome.cshlp.org/content/9/11/1059.abstract 
AB The short arm of human chromosome 21 (21p) contains many different types of repetitive sequences and is highly homologous to the short arms of other acrocentric chromosomes. Owing to its repetitive nature and the lack of chromosome 21p-specific molecular markers, most physical maps of chromosome 21 exclude this region. We constructed a physical map of chromosome 21p using sequence tagged site (STS) content mapping of yeast artificial chromosomes (YACs). To this end, 39 STSs located on the short arm or near the centromere of chromosome 21 were constructed, including four polymorphic simple tandem repeats (STRs) and two expressed sequence tags (ESTs). Thirty YACs were selected from the St. Louis YAC library, the chromosome 21-enriched ICRF YAC library, and the CEPH YAC and megaYAC libraries. These were assembled in a YAC contig map ranging from the centromere to the rDNA gene cluster at 21p12. The total size of the region covered by YACs is estimated between 2.9 and 5 Mb. The integrity of the YAC contig was confirmed by restriction enzyme fingerprinting and fluorescence in situ hybridization (FISH). One gap with an estimated size of 400 kb remained near the telomeric end of the contig. This YAC contig map of the short arm of human chromosome 21 constitutes a basic framework for further structural and functional studies of chromosome 21p.