TY  - JOUR
A1  - Cheng, Suzanne
A1  - Grow, Michael A.
A1  - Pallaud, Céline
A1  - Klitz, William
A1  - Erlich, Henry A.
A1  - Visvikis, Sophia
A1  - Chen, John J.
A1  - Pullinger, Clive R.
A1  - Malloy, Mary J.
A1  - Siest, Gérard
A1  - Kane, John P.
T1  - A Multilocus Genotyping Assay for Candidate Markers of Cardiovascular Disease Risk
Y1  - 1999/10/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 936 
EP  - 949 
DO  - 10.1101/gr.9.10.936 
VL  - 9 
IS  - 10 
UR  - http://genome.cshlp.org/content/9/10/936.abstract 
N2  - A number of chronic diseases, including cardiovascular disease, appear to have a multifactorial genetic risk component. Consequently, techniques are needed to facilitate evaluation of complex genetic risk factors in large cohorts. We have designed a prototype assay for genotyping a panel of 35 biallelic sites that represent variation within 15 genes from biochemical pathways implicated in the development and progression of cardiovascular disease. Each DNA sample is amplified using two multiplex polymerase chain reactions, and the alleles are genotyped simultaneously using an array of immobilized, sequence-specific oligonucleotide probes. This multilocus assay was applied to two types of cohorts. Population frequencies for the markers were estimated using 496 unrelated individuals from a family-based cohort, and the observed values were consistent with previous reports. Linkage disequilibrium between consecutive pairs of markers within theapoCIII, LPL, and ELAM genes was also estimated. A preliminary analysis of single and pairwise locus associations with severity of atherosclerosis was performed using a composite cohort of 142 individuals for whom quantitative angiography data were available; evaluation of the potentially interesting associations observed will require analysis of an independent and larger cohort. This assay format provides a research tool for studies of multilocus genetic risk factors in large cardiovascular disease cohorts, and for the subsequent development of diagnostic tests. 
ER  -