RT Journal
A1 Centola, Michael
A1 Chen, Xiaoguang
A1 Sood, Raman
A1 Deng, Zuoming
A1 Aksentijevich, Ivona
A1 Blake, Trevor
A1 Ricke, Darrell O.
A1 Chen, Xiang
A1 Wood, Geryl
A1 Zaks, Nurit
A1 Richards, Neil
A1 Krizman, David
A1 Mansfield, Elizabeth
A1 Apostolou, Sinoula
A1 Liu, Jingmei
A1 Shafran, Neta
A1 Vedula, Anil
A1 Hamon, Melanie
A1 Cercek, Andrea
A1 Kahan, Tanaz
A1 Gumucio, Deborah
A1 Callen, David F.
A1 Richards, Robert I.
A1 Moyzis, Robert K.
A1 Doggett, Norman A.
A1 Collins, Francis S.
A1 Liu, P. Paul
A1 Fischel-Ghodsian, Nathan
A1 Kastner, Daniel L.
T1 Construction of an ∼700-kb Transcript Map Around the Familial Mediterranean Fever Locus on Human Chromosome 16p13.3
JF Genome Research 
JO Genome Research 
YR 1998 
FD November 01 
VO 8 
IS 11 
SP 1172 
OP 1191 
DO 10.1101/gr.8.11.1172 
UL http://genome.cshlp.org/content/8/11/1172.abstract 
AB We used a combination of cDNA selection, exon amplification, and computational prediction from genomic sequence to isolate transcribed sequences from genomic DNA surrounding the familial Mediterranean fever (FMF) locus. Eighty-seven kb of genomic DNA around D16S3370, a marker showing a high degree of linkage disequilibrium with FMF, was sequenced to completion, and the sequence annotated. A transcript map reflecting the minimal number of genes encoded within the ∼700 kb of genomic DNA surrounding the FMF locus was assembled. This map consists of 27 genes with discreet messages detectable on Northerns, in addition to three olfactory-receptor genes, a cluster of 18 tRNA genes, and two putative transcriptional units that have typical intron–exon splice junctions yet do not detect messages on Northerns. Four of the transcripts are identical to genes described previously, seven have been independently identified by the French FMF Consortium, and the others are novel. Six related zinc-finger genes, a cluster of tRNAs, and three olfactory receptors account for the majority of transcribed sequences isolated from a 315-kb FMF central region (betweenD16S468/D16S3070 and cosmid 377A12). Interspersed among them are several genes that may be important in inflammation. This transcript map not only has permitted the identification of the FMF gene (MEFV), but also has provided us an opportunity to probe the structural and functional features of this region of chromosome 16.